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Genetic Insert: MAVS-M1A,M142A
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529381
Comments: Has extra restriction sites upstream of MAVS start codon. HindIII and XhoI
Proper citation: RRID:Addgene_52018 Copy
Species: Synthetic
Genetic Insert: Yellow Cameleon-Nano15
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693999
Comments: GenBank accession number: GU071083
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51961 Copy
Species: Synthetic
Genetic Insert: Yellow Cameleon-Nano30
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693999
Comments: GenBank accession number: GU071084
Proper citation: RRID:Addgene_51963 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492. The improved brightness of Nano-lantern should generate power densities in the range of 1μW/cm2 (versus 0.1μW/cm2 for RLuc8) following transient overexpression in the micromolar range in human cells, and thus increase imaging potential.
Proper citation: RRID:Addgene_51969 Copy
Species: Homo sapiens
Genetic Insert: CD36
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 102
Proper citation: RRID:Addgene_52025 Copy
Species: Homo sapiens
Genetic Insert: ARMET
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 118
Proper citation: RRID:Addgene_52026 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51971 Copy
Species: Homo sapiens
Genetic Insert: HA-Daxx
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23405218
Proper citation: RRID:Addgene_52023 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 71
Proper citation: RRID:Addgene_52022 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-2GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859494
Proper citation: RRID:Addgene_51979 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492
The minor discrepancies between the QC and depositor sequence have no functional consequence and Addgene's QC sequence should be used for comparison.
Please note the upstream KpnI site shown on the depositor's map is not present in this plasmid.
Proper citation: RRID:Addgene_51975 Copy
Species: Homo sapiens
Genetic Insert: CTGF
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 121
Proper citation: RRID:Addgene_52028 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859498
Proper citation: RRID:Addgene_51980 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24305048
Proper citation: RRID:Addgene_52036 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-2G
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859496
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51982 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859495
Proper citation: RRID:Addgene_51981 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24305048
Comments: let-7 sequence for nucleotides 2-15 is CAACCTACTACCTCA.
Proper citation: RRID:Addgene_52035 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid 1497); Backbone Size:4406; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23805378
Comments: The D->V point mutation in DAF-16 has no functional consequence.
Proper citation: RRID:Addgene_52032 Copy
Species: Homo sapiens
Genetic Insert: FGG
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 83
Co-transfect with FGA-bio-His (Addgene 52029) and FGB (Addgene 52030) to create the fibrinogen trimer
Proper citation: RRID:Addgene_52031 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_ATP0
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN974906. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51989 Copy
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