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Species: Pseudomonas putida DOT-T1E
Genetic Insert: single-stranded DNA recombineering
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_199241 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205293 Copy
Species: Scytonema hofmannii
Genetic Insert: ShTnsB, ShTnsC, ShTniQ, ShCas12k
Vector Backbone Description: Backbone Size:3359; Vector Backbone:pR01600; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37368499
Comments: The vector backbone was derived from plasmid pFlpe2 from
Choi, K. H., Mima, T., Casart, Y., Rholl, D., Kumar, A., Beacham, I. R., & Schweizer, H. P. (2008). Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Applied and environmental microbiology, 74(4), 1064–1075. https://doi.org/10.1128/AEM.02430-07
Proper citation: RRID:Addgene_180076 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2545; Vector Backbone:BASIC_SEVA_69.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36381610
Proper citation: RRID:Addgene_194214 Copy
Vector Backbone Description: Vector Backbone:pProUSER63H0G; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Proper citation: RRID:Addgene_191426 Copy
Vector Backbone Description: Backbone Size:3663; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS with FLAG epitope integrated at SmaI site, confers gentamycin resistance
Proper citation: RRID:Addgene_225162 Copy
Species: Pseudomonas aeruginosa SG17M
Genetic Insert: tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
Vector Backbone Description: Backbone Size:6055; Vector Backbone:pJN105; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38176647
Proper citation: RRID:Addgene_214260 Copy
Vector Backbone Description: Backbone Marker:Ichiro Matsumura, Addgene plasmid #26702; Backbone Size:2515; Vector Backbone:pBAV1K-T5-gfp; Vector Types:Unspecified; Bacterial Resistance:Gentamicin
Comments: Use Gentamicin 15 µg/mL in E. coli, Gentamicin 30 µg/mL in Synechococcus sp. PCC 7002
Grow at 30°C in E. coli, grow at 37°C in Synechococcus sp. PCC 7002
For increased transformation into PCC 7002, miniprep this plasmid from E.coli strain DHIP1.
DHIP1 deposited under the following paper:
Kamoku C, Nielsen DR. Highly Iterated Palindrome 1 (HIP1) sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Published online September 10, 2024:2024.09.10.611283. doi:10.1101/2024.09.10.611283
Please visit https://doi.org/10.1101/2024.07.31.606084 for bioRxiv preprint.
Proper citation: RRID:Addgene_226658 Copy
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229955 Copy
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229954 Copy
Species: Homo sapiens
Genetic Insert: ActBT2ATetP2AP - H
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229972 Copy
Species: Homo sapiens
Genetic Insert: ActBT2ATetP2AP - mAGH
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229973 Copy
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229956 Copy
Species: Homo sapiens
Genetic Insert: mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Addgene's NGS results found F394L mutation in EYFP-tubulin that does not affect plasmid function.
Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229974 Copy
Species: Homo sapiens
Genetic Insert: mito-mCherry
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229966 Copy
Species: Homo sapiens
Genetic Insert: H2B iRFP720
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229968 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39919271
Proper citation: RRID:Addgene_231319 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39919271
Proper citation: RRID:Addgene_231318 Copy
Species: Synthetic
Genetic Insert: SOX (K250A)
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:3400; Vector Backbone:pACEmam1; Vector Types:Unspecified; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36537232
Proper citation: RRID:Addgene_192052 Copy
Species: Synthetic
Genetic Insert: SOX (D221N E244Q)
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:3400; Vector Backbone:pACEmam1; Vector Types:Unspecified; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36537232
Proper citation: RRID:Addgene_192053 Copy
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