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Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65592 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65595 Copy
Species: E. coli
Genetic Insert: accA
Vector Backbone Description: Backbone Size:5149; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_66866 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83385 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83386 Copy
Species: E. coli
Genetic Insert: sucCD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:pET42b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_83324 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28277608
Proper citation: RRID:Addgene_83392 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28277608
Proper citation: RRID:Addgene_83393 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83397 Copy
Species: E. coli
Genetic Insert: Ivy
Vector Backbone Description: Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23220215
Proper citation: RRID:Addgene_83920 Copy
Species: E. coli
Genetic Insert: Gro EL/ES
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23220215
Proper citation: RRID:Addgene_83923 Copy
Species: E. coli
Genetic Insert: E. coli RecA promoter
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30388413
Proper citation: RRID:Addgene_84722 Copy
Species: E. coli
Genetic Insert: E. coli RecA promoter
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30388413
Proper citation: RRID:Addgene_84721 Copy
Species: E. coli
Genetic Insert: E. coli RecA promoter
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30388413
Proper citation: RRID:Addgene_84726 Copy
Species: E. coli
Genetic Insert: E. coli RecA promoter
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30388413
Proper citation: RRID:Addgene_84725 Copy
Species: E. coli
Genetic Insert: E. coli RecA promoter
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30388413
Proper citation: RRID:Addgene_84719 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~2 minutes. See author's map.
Proper citation: RRID:Addgene_8818 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal >20 hours. See author's map.
Proper citation: RRID:Addgene_8816 Copy
Species: E. coli
Genetic Insert: Streptavidin-K121R-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757182
Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3)
In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)
Proper citation: RRID:Addgene_89880 Copy
Species: E. coli
Genetic Insert: Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757182
Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3)
In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)
Proper citation: RRID:Addgene_89881 Copy
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