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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCWX-R4DESTR2-PGK-PURO Resource Report Resource Website |
RRID:Addgene_114319 | Chloramphenicol and Ampicillin | This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R | Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:02:22 | 0 | ||||
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pCLXEBR-pTF-kRasV12 Resource Report Resource Website 1+ mentions |
RRID:Addgene_114318 | Ras | Homo sapiens | Ampicillin | Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. Subcloned from Addgene Plasmid #31200. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al. | Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | G12V oncogenic mutation. Constitutively active K-ras protein. | 2026-08-15 01:02:21 | 1 | |
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pCLXEBR-pTF-mBmi1 Resource Report Resource Website |
RRID:Addgene_114317 | mBmi1 | Mus musculus | Ampicillin | PMID:23097121 | Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al. | Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:21 | 0 | |
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pCWX-UBI-hTert-PGK-BSD Resource Report Resource Website 1+ mentions |
RRID:Addgene_114316 | hTERT | Homo sapiens | Ampicillin | PMID:23097121 | Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. | Vector Backbone:pCWX-PGK-BSD; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:22 | 1 | |
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pWPI-TFDP2 Resource Report Resource Website |
RRID:Addgene_114300 | TFDP2 | Homo sapiens | Ampicillin | PMID:23907129 | Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. | Vector Backbone:pWPI-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:21 | 0 | |
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pCWXPG-UBI-Ngn3PDX1MafA Resource Report Resource Website |
RRID:Addgene_114303 | Ngn3 | Homo sapiens | Ampicillin | PMID:21761975 | Vector Backbone:pCWXPG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Ngn3, PDX1 and MafA proteins are expressed from a precursor with self-cleavable 2A peptides | 2026-08-15 01:02:21 | 0 | |
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pCWXPG-UBI-MafA Resource Report Resource Website |
RRID:Addgene_114302 | MafA | Homo sapiens | Ampicillin | PMID:21761975 | Vector Backbone:pCWXPG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:21 | 0 | ||
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pCLXEBR-pTF-Ngn3 Resource Report Resource Website |
RRID:Addgene_114307 | Ngn3 | Homo sapiens | Ampicillin | PMID:21761975 | pTF is an optimized TET-promoter, described in HGT 2011, Giry et al. | Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:21 | 0 | |
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pCLX-Ngn3-GFP Resource Report Resource Website |
RRID:Addgene_114309 | GFP | Homo sapiens | Ampicillin | Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:21 | 0 | |||
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pGEX-gp78c/2767 Resource Report Resource Website 1+ mentions |
RRID:Addgene_11429 | gp78c | Homo sapiens | Ampicillin | PMID:11724934 | Backbone Marker:Amersham/GE; Backbone Size:5000; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | n/a | 2026-08-15 01:02:21 | 1 | |
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Anti-VGlut3 [N34/34.2R] Resource Report Resource Website |
RRID:Addgene_114494 | anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750780. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:24 | 0 | |
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Anti-NGL-3/LRRC4B [N51/6.2R] Resource Report Resource Website |
RRID:Addgene_114493 | anti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750799. Isotype: IgG2a. Additional species: rat, mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:23 | 0 | |
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AICSDP-49: MYL2-mEGFP Resource Report Resource Website |
RRID:Addgene_114414 | MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette | Homo sapiens | Ampicillin | PMID:30956114 | This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human MYL2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint. | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2026-08-15 01:02:22 | 0 |
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AICSDP-34: TNNI1-mEGFP Resource Report Resource Website |
RRID:Addgene_114411 | TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette | Homo sapiens | Ampicillin | PMID:30956114 | This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint. | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:22 | 0 | |
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iLID-eGFP-2xC2-iLID Resource Report Resource Website |
RRID:Addgene_114418 | iLID domain | Synthetic | Kanamycin | PMID:30182785 | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:22 | 0 | ||
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iLID-eGFP-3xC2 Resource Report Resource Website |
RRID:Addgene_114417 | iLID domain | Synthetic | Kanamycin | PMID:30182785 | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:22 | 0 | ||
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iLID-eGFP-C2 Resource Report Resource Website |
RRID:Addgene_114415 | iLID domain | Synthetic | Kanamycin | PMID:30182785 | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:22 | 0 | ||
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pAAVS1-TRE-MA4-GFP Resource Report Resource Website |
RRID:Addgene_114380 | MLL-AF4 fusion gene | Homo sapiens | Ampicillin | PMID:29386396 | Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:22 | 0 | ||
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Anti-Synaptotagmin-12 [N277/7R] Resource Report Resource Website |
RRID:Addgene_114482 | anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750763. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:23 | 0 | |
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Anti-SUR2B [N323B/20R] Resource Report Resource Website |
RRID:Addgene_114480 | anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750775. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:02:23 | 0 |
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