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pSH624-ssr
 
Resource Report
Resource Website 		RRID:Addgene_199241 single-stranded DNA recombineering Pseudomonas putida DOT-T1E Gentamicin PMID:37798358 Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin 2026-08-15 01:27:52 0
pHelper_NoMutL_V2_gentR
 
Resource Report
Resource Website 		RRID:Addgene_205293 CspRecT / Bxb-1 Synthetic Gentamicin Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin 2026-08-15 01:27:54 0
pRO16Sh5
 
Resource Report
Resource Website 		RRID:Addgene_180076 ShTnsB, ShTnsC, ShTniQ, ShCas12k Scytonema hofmannii Gentamicin PMID:37368499 The vector backbone was derived from plasmid pFlpe2 from Choi, K. H., Mima, T., Casart, Y., Rholl, D., Kumar, A., Beacham, I. R., & Schweizer, H. P. (2008). Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Applied and environmental microbiology, 74(4), 1064–1075. https://doi.org/10.1128/AEM.02430-07 Backbone Size:3359; Vector Backbone:pR01600; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin 2026-08-15 01:27:13 0
BASIC_SEVA_69.11
 
Resource Report
Resource Website 		RRID:Addgene_194214 mScarlet counter-selection cassette Synthetic Gentamicin PMID:36381610 Backbone Size:2545; Vector Backbone:BASIC_SEVA_69.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin 2026-08-15 01:27:16 0
pProUSER63H0G
 
Resource Report
Resource Website 		RRID:Addgene_191426 Gentamicin Vector Backbone:pProUSER63H0G; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin 2026-08-15 01:24:28 0
pHBJT011
 
Resource Report
Resource Website 		RRID:Addgene_225162 Gentamicin PMID:39244484 Low copy plasmid (pSC101 ori and repA), pUC19 MCS with FLAG epitope integrated at SmaI site, confers gentamycin resistance Backbone Size:3663; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin 2026-08-15 01:30:42 0
pSG069
 
Resource Report
Resource Website 		RRID:Addgene_214260 tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M Pseudomonas aeruginosa SG17M Gentamicin PMID:38176647 Backbone Size:6055; Vector Backbone:pJN105; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin 2026-08-15 01:30:49 0
pCMK191
 
Resource Report
Resource Website 		RRID:Addgene_226658 Gentamicin Use Gentamicin 15 µg/mL in E. coli, Gentamicin 30 µg/mL in Synechococcus sp. PCC 7002 Grow at 30°C in E. coli, grow at 37°C in Synechococcus sp. PCC 7002 For increased transformation into PCC 7002, miniprep this plasmid from E.coli strain DHIP1. DHIP1 deposited under the following paper: Kamoku C, Nielsen DR. Highly Iterated Palindrome 1 (HIP1) sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Published online September 10, 2024:2024.09.10.611283. doi:10.1101/2024.09.10.611283 Please visit https://doi.org/10.1101/2024.07.31.606084 for bioRxiv preprint. Backbone Marker:Ichiro Matsumura, Addgene plasmid #26702; Backbone Size:2515; Vector Backbone:pBAV1K-T5-gfp; Vector Types:Unspecified; Bacterial Resistance:Gentamicin 2026-08-15 01:31:51 0
psLIB TRE
 
Resource Report
Resource Website 		RRID:Addgene_229955 Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:52 0
psLIB EF1alpha
 
Resource Report
Resource Website 		RRID:Addgene_229954 Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:52 0
psBIG1a ActBT2ATetP2AP - H
 
Resource Report
Resource Website 		RRID:Addgene_229972 ActBT2ATetP2AP - H Homo sapiens Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:52 0
psBIG1a ActBT2ATetP2AP - mAGH
 
Resource Report
Resource Website 		RRID:Addgene_229973 ActBT2ATetP2AP - mAGH Homo sapiens Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:52 0
psLIB U6
 
Resource Report
Resource Website 		RRID:Addgene_229956 Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:54 0
pDONOR 6colors
 
Resource Report
Resource Website 		RRID:Addgene_229974 mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin Homo sapiens Gentamicin Addgene's NGS results found F394L mutation in EYFP-tubulin that does not affect plasmid function. Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin See Depositor Comments 2026-08-15 01:31:54 0
psLIB TRE mito-mCherry
 
Resource Report
Resource Website 		RRID:Addgene_229966 mito-mCherry Homo sapiens Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:54 0
psLIB TRE H2B iRFP720
 
Resource Report
Resource Website 		RRID:Addgene_229968 H2B iRFP720 Homo sapiens Gentamicin Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-08-15 01:31:54 0
pNDGG050
 
Resource Report
Resource Website 		RRID:Addgene_231319 Gentamicin PMID:39919271 Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin 2026-08-15 01:32:12 0
pNDGG049
 
Resource Report
Resource Website 		RRID:Addgene_231318 Gentamicin PMID:39919271 Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin 2026-08-15 01:32:12 0
pACE SOX (ORF37) K250A
 
Resource Report
Resource Website 		RRID:Addgene_192052 SOX (K250A) Synthetic Gentamicin PMID:36537232 Backbone Marker:Geneva Biotech; Backbone Size:3400; Vector Backbone:pACEmam1; Vector Types:Unspecified; Bacterial Resistance:Gentamicin DNA binding mutant K250A 2026-08-15 01:31:25 0
pACE SOX (ORF37) D221N E244Q
 
Resource Report
Resource Website 		RRID:Addgene_192053 SOX (D221N E244Q) Synthetic Gentamicin PMID:36537232 Backbone Marker:Geneva Biotech; Backbone Size:3400; Vector Backbone:pACEmam1; Vector Types:Unspecified; Bacterial Resistance:Gentamicin 2X catalytic mutant D221N E244Q 2026-08-15 01:31:25 0

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