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Plasmids are provided by Addgene and DGRC.

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  • Organism:e. coli (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
HL 818 (=HL 716 + pHL 98)
 
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RRID:Addgene_30006 pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:colE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:21 0
HL 839 (=HL 716 + pHL 108)
 
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RRID:Addgene_30008 pLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:23 0
HL 3375 (=HL 865 + pHL 1008)
 
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RRID:Addgene_30072 pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:22 0
HL 3357 (=HL 716 + pHL 1191)
 
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RRID:Addgene_30068 pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 3347 (=HL 3338 + pHL 720)
 
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RRID:Addgene_30067 pLtetO-1:mCherry, pLlacO-1:sodB::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 3327 (=HL 716 + pHL 1009)
 
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RRID:Addgene_30060 pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 3186 (=HL 716 + pHL 282)
 
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RRID:Addgene_30051 pLtetO-1:mCherry, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 3152 (=HL 716 + pHL 1056)
 
Resource Report
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RRID:Addgene_30048 pCon:MicC, pLlacO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 3151 (=HL 716 + pHL 1037)
 
Resource Report
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RRID:Addgene_30047 pLtetO-1:DsrA, pCon:MicC, pLlacO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:21 0
HL 4178 (=HL 862 + pHL 1360)
 
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RRID:Addgene_30097 pLlacO-1:hfq, pLtetO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 3859 (=HL 716 + pHL 1305)
 
Resource Report
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RRID:Addgene_30089 pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:22 0
HL 4202 (=HL 716 + pHL 1389)
 
Resource Report
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RRID:Addgene_30100 pLtetO-1:DsrA, pLlacO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 0
HL 4218 (=HL 716 + pHL 1411)
 
Resource Report
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RRID:Addgene_30105 pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:22 0
HL 4212 (=HL 716 + pHL 1414)
 
Resource Report
Resource Website
RRID:Addgene_30104 pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:13:25 0
pET24c(+)-EFTs
 
Resource Report
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RRID:Addgene_31173 E. coli elongation factor Ts E. coli Kanamycin PMID:8765000 Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:32 0
pDL52 (clone45)
 
Resource Report
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RRID:Addgene_188578 ribA E. coli Kanamycin PMID:35863619 Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels. Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimization, synthetic ribosome binding sites 2026-08-15 01:23:43 0
pDL52 (clone76)
 
Resource Report
Resource Website
RRID:Addgene_188579 ribA E. coli Kanamycin PMID:35863619 Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels. Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimization, synthetic ribosome binding sites 2026-08-15 01:23:41 0
pSelect-6
 
Resource Report
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RRID:Addgene_190243 LacI E. coli Spectinomycin PMID:35657952 Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:23:48 0
pSBK.012
 
Resource Report
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RRID:Addgene_187212 Retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC]) E. coli Kanamycin PMID:35896746 P35Q mutation in Cas2 insert does not affect plasmid function. Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint. Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:23:49 0
pSBK.010
 
Resource Report
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RRID:Addgene_187210 Retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]) E. coli Kanamycin PMID:35896746 Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint. Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:23:51 0

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