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http://www.addgene.org/165441

Species: Other
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Marker:Roger Tsien; Vector Backbone:AAV-flex-ReaChR-citrine (Addgene 50955); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34176926

Proper citation: RRID:Addgene_165441 Copy   

•   Source: Addgene

http://www.addgene.org/165502

Species: Other
Genetic Insert: 9 degrees N-7 DNA Polymerase chain terminating
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: 9 Degrees North DNA polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides. Comparable to Therminator DNA Pol at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165502 Copy   

•   Source: Addgene

http://www.addgene.org/165503

Species: Other
Genetic Insert: DNA Ligase IV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction. Also involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165503 Copy   

•   Source: Addgene

http://www.addgene.org/165508

Species: Other
Genetic Insert: HindIII
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165508 Copy   

•   Source: Addgene

http://www.addgene.org/165457

Species: Other
Genetic Insert: CRE
Vector Backbone Description: Backbone Size:10785; Vector Backbone:AAVS1-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33765444

Proper citation: RRID:Addgene_165457 Copy   

•   Source: Addgene

http://www.addgene.org/165562

Species: Other
Genetic Insert: Pwo DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase; additionally exhibits 3' to 5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165562 Copy   

•   Source: Addgene

http://www.addgene.org/165567

Species: Other
Genetic Insert: XbaI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165567 Copy   

•   Source: Addgene

http://www.addgene.org/165568

Species: Other
Genetic Insert: NcoI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165568 Copy   

•   Source: Addgene

http://www.addgene.org/165565

Species: Other
Genetic Insert: RNAse A
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA. Effectively cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide which forms a 2',3'-cyclic phosphate which is then hydrolyzed to the corresponding 3'-nucleoside phosphate. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165565 Copy   

•   Source: Addgene

http://www.addgene.org/165566

Species: Other
Genetic Insert: Thermus thermophilus (Tth) RT
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165566 Copy   

•   Source: Addgene

http://www.addgene.org/165560

Species: Other
Genetic Insert: T4 gene 32
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165560 Copy   

•   Source: Addgene

http://www.addgene.org/165569

Species: Other
Genetic Insert: Tth DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: High fidelity version Taq DNA Ligase. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165569 Copy   

•   Source: Addgene

http://www.addgene.org/165550

Species: Other
Genetic Insert: T4 PGD (T4 Endonuclease V)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases cis-syn cyclobutane pyrimidine dimers, including T^T, T^C and C^C, generating an AP site. The AP lyase activity cleaves an AP site via beta-elimination, creating a 1 nucleotide DNA gap with 3'-alpha, beta-unsaturated aldehyde and 5'-phosphate termini. Also known as T4 Endonuclease V. This enzyme in combination with other enzymes from E. coli (UDG, fpg, Endonuclease IV, Endonuclease VIII), Bst-DNA pol and Taq/Tth-DNA Ligase forms the proprietary NEB Enzyme mix preCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165550 Copy   

•   Source: Addgene

http://www.addgene.org/165551

Species: Other
Genetic Insert: Endonuclease IV E. coli
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Used in the preCR mix from NEB; DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165551 Copy   

•   Source: Addgene

http://www.addgene.org/165556

Species: Other
Genetic Insert: Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165556 Copy   

•   Source: Addgene

http://www.addgene.org/165558

Species: Other
Genetic Insert: M.EcoRV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by EcoRV. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165558 Copy   

•   Source: Addgene

http://www.addgene.org/165575

Species: Other
Genetic Insert: RNAse H
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus. Along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis, and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA pre-primer. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165575 Copy   

•   Source: Addgene

http://www.addgene.org/165572

Species: Other
Genetic Insert: Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165572 Copy   

•   Source: Addgene

http://www.addgene.org/165576

Species: Other
Genetic Insert: T3 RNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165576 Copy   

•   Source: Addgene

http://www.addgene.org/165577

Species: Other
Genetic Insert: M.XbaI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165577 Copy   

•   Source: Addgene