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Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19266305
Comments: driven by Xenopus laevis heat shock 70 (hsp 70) promoter
Proper citation: RRID:Addgene_30521 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase tdTomato
Vector Backbone Description: Backbone Marker:Genbank DQ836146; Backbone Size:3007; Vector Backbone:I-SceI-pBSII-SK+; Vector Types:Flp/FRT, heat shock inducible, I-SceI Megabase vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19266305
Comments: FLP driven by Xenopus laevis heat shock 70 (hsp 70) promoter with CMV driven tdTomato, both in I-SceI megabase vector
Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.
Proper citation: RRID:Addgene_30523 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ulp1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2894; Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19031436
Comments: Mossessova E, Lima CD. Mol Cell. 2000 May;5(5):865-76.
Note: pFGET19_Ulp1 (#64697) is a new version of pHYRS52(#31122) with kanamycin resistance.
Proper citation: RRID:Addgene_31122 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP9
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9427760
Proper citation: RRID:Addgene_31722 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cofilin
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17875745
Comments: The GFP-cofilin construct has S65T-GFP and a 12–amino acid linker (GHGTGSTGSGSS) inserted in between amino acids N74 and G75 of cofilin. Note that the GFP is NOT N-terminal.
A missense mutation was detected by the lab in sequencing: converting the third glycine in the 5′ GFP linker into an arginine. This was the construct used in the paper.
Proper citation: RRID:Addgene_31843 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cofilin
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17875745
Comments: The GFP-cofilin construct has S65T-GFP and a 12–amino acid linker (GHGTGSTGSGSS) inserted in between amino acids N74 and G75 of cofilin. Note that the GFP is NOT N-terminal.
A missense mutation was detected by the lab in sequencing: converting the third glycine in the 5′ GFP linker into an arginine. This was the construct used in the paper.
Proper citation: RRID:Addgene_31844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flippase2::PEST
Vector Backbone Description: Vector Backbone:pJFRC4-3XUAS-IVS-mCD8::GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21831835
Proper citation: RRID:Addgene_32133 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5450; Vector Backbone:pET-30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_32178 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5450; Vector Backbone:pET-30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_32179 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: 2 µm S. cerevisiae replication
Proper citation: RRID:Addgene_32164 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: 2 µm S. cerevisiae replication
Proper citation: RRID:Addgene_32165 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: 2 µm S. cerevisiae replication.
Proper citation: RRID:Addgene_32169 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.
I44A mutation introduced by quickchange of LHP308 (Addgene plasmid 32151). Entire Ub insert was sequenced - no other mutation found.
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32163 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.
K48R mutation introduced by quickchange of LHP308 (Addgene plasmid 32151).
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659916
Comments: BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32151 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pTER62; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659916
Comments: Ub-no Lys; CUP1-Ub K6R, K11R, K27R, K29R, K33A, K48R, K63R (all Lys Changed to Arg); BamHI-BglII UBI fragment from YEp96 (D. Ecker, U PENN) was cloned into pTER62 (Mike Ellison, University of Alberta) cut with BamHI and BglII.
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32150 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hsFlp1
Vector Backbone Description: Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21831835
Proper citation: RRID:Addgene_32148 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS8
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5000; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32241 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: INP51
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:PCplac22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: HA tag is likely 3xHA
Proper citation: RRID:Addgene_32240 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: INP53
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:YEplac181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32198 Copy
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