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Species: Homo sapiens
Genetic Insert: mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108137 Copy
Species: Homo sapiens
Genetic Insert: mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108136 Copy
Species: Homo sapiens
Genetic Insert: mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]6:SACM1L(521-587)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108135 Copy
Species: Homo sapiens
Genetic Insert: mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]4:SACM1L(521-587)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108134 Copy
Species: Homo sapiens
Genetic Insert: mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Dep confirmed that Y434F is a common point mutation that does not affect plasmid function. Updated plasmid information.
Proper citation: RRID:Addgene_108133 Copy
Species: Homo sapiens
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108107 Copy
Species: Homo sapiens
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108104 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12505989
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a L520P mutation when compared to GenBank reference sequence NP_002733.1. Although the effect of this mutation is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10808 Copy
Species: Homo sapiens
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108103 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12637538
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a Myc tag inserted between amino acids 33&34, Q66R, F139S and G877W mutations when compared to GenBank reference sequence NP_002733.1. Although the effect of these mutations is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10809 Copy
Species: Homo sapiens
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108108 Copy
Species: Homo sapiens
Genetic Insert: LKB1
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108111 Copy
Species: Homo sapiens
Genetic Insert: PKD1 RNAi
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12505989
Comments: Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCATG CTGTGGGGGC TGGTACTTCA AGAGAGTACC AGCCCCCACA GCATTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAATGCTG TGGGGGCTGG TACTCTCTTG AAGTACCAGC CCCCACAGCA TGGG-3'.
Proper citation: RRID:Addgene_10815 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15024053
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a L520P mutation when compared to GenBank reference sequence NP_002733.1. Although the effect of this mutation is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10814 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12637538
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains F94I, V410G and D791G mutations when compared to GenBank reference sequence NP_002733.1. Although the effect of these mutation are unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10811 Copy
Species: Homo sapiens
Genetic Insert: Drosha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5017; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15589161
Proper citation: RRID:Addgene_10828 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5313; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10821 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 4
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10824 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5310; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10823 Copy
Species: Homo sapiens
Genetic Insert: EYFP-p23
Vector Backbone Description: Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17010336
Proper citation: RRID:Addgene_108225 Copy
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