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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pU6Rosa-CAG-Gal4-Rad18UBD
 
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RRID:Addgene_119012 Gal4 Saccharomyces cerevisiae Ampicillin PMID:33097066 Backbone Size:2961; Vector Backbone:Bluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:09 0
pYS104
 
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RRID:Addgene_1190 Hsp104 Saccharomyces cerevisiae Ampicillin PMID:8662547 Backbone Marker:Hieter; Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wild-type Hsp104 sequence with 588bp of 5' promoter region included. 2026-08-15 01:03:09 0
pK218T
 
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RRID:Addgene_1193 Hsp104 Saccharomyces cerevisiae Ampicillin PMID:8662547 Backbone Marker:Hieter; Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed lysine 218 to threonine 2026-08-15 01:03:12 0
pK620T
 
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RRID:Addgene_1194 Hsp104 Saccharomyces cerevisiae Ampicillin PMID:8662547 Backbone Marker:Hieter; Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed lysine 620 to threonine 2026-08-15 01:03:12 0
pGRE NPD-GFP
 
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RRID:Addgene_1197 N-terminus of Sup35 fused to GFP Saccharomyces cerevisiae Ampicillin PMID:8662547 2 micron yeast plasmid Please note that BamHI and SpeI are NOT single cutters in this plasmid. See the Addgene digest above. Backbone Size:2800; Vector Backbone:pUC18 w/ GRE promoter; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin fused N-terminal Sup35 (~1-253aa) to GFP 2026-08-15 01:03:14 0
sup35-R320I
 
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RRID:Addgene_1199 Sup35p Saccharomyces cerevisiae Ampicillin PMID:15311209 yeast integration plasmid for replacement of Sup35 with mutant. Cut with AgeI for integration. Additional Reference: Bradley ME, Bagriantsev S, Vishveshwara N and Liebman SW. Guanidine reduces stop codon read-through caused by missense mutations in SUP35 or SUP45. Yeast 20, 625-632 (2003). Backbone Marker:Hieter; Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed arg 320 to isoleucine 2026-08-15 01:03:16 0
sup35-C653R
 
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RRID:Addgene_1198 Sup35p Saccharomyces cerevisiae Ampicillin PMID:15311209 yeast integration plasmid for replacement of Sup35 with mutant. Cut with AgeI for integration. Additional Reference: Bradley ME, Bagriantsev S, Vishveshwara N and Liebman SW. Guanidine reduces stop codon read-through caused by missense mutations in SUP35 or SUP45. Yeast 20, 625-632 (2003). Backbone Marker:Hieter; Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed cys 653 to arg 2026-08-15 01:03:17 0
pRS313_ATP3G919C
 
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RRID:Addgene_120255 ATP3 Saccharomyces cerevisiae Ampicillin PMID:34799698 Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin G919 changed to C; Alanine 307 changed to Proline 2026-08-15 01:03:22 0
pRS313_ATP3mut
 
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RRID:Addgene_120256 ATP3 Saccharomyces cerevisiae Ampicillin PMID:34799698 Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Isoleucine 304 changed to Asparagine; Alanine 307 changed to Proline 2026-08-15 01:03:20 0
pTEF-Sup35C
 
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1+ mentions
RRID:Addgene_1202 Sup35 C-terminus Saccharomyces cerevisiae Ampicillin PMID:15311209 yeast integration plasmid with insert under TEF promoter Backbone Marker:Hieter; Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Sup35 C-terminus ~254aa-end 2026-08-15 01:03:19 2
pNMG
 
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RRID:Addgene_1203 NM region of Sup35 Saccharomyces cerevisiae Ampicillin PMID:15311209 yeast integration plasmid with NM-GFP under TEF promoter. For integration at URA3 cut with StuI Backbone Marker:Hieter; Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NM region (1-253aa) of Sup35 2026-08-15 01:03:21 0
6969 pNOTAGHsp104(K620T)
 
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1+ mentions
RRID:Addgene_1237 Hsp104 (K620T) Saccharomyces cerevisiae Ampicillin PMID:15155912 Backbone Size:6526; Vector Backbone:pNOTAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K620T 2026-08-15 01:03:57 1
6011 pETH6K620Tb
 
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RRID:Addgene_1240 Hsp104 (K620T mutant) Saccharomyces cerevisiae Kanamycin PMID:15155912 Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin K620T 2026-08-15 01:04:01 0
pET29b-IPP1-His
 
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1+ mentions
RRID:Addgene_124137 Inorganic pyrophosphatase Saccharomyces cerevisiae Kanamycin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:03 3
6107 pETH6KT218
 
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RRID:Addgene_1241 Hsp104 (K218T) Saccharomyces cerevisiae Kanamycin PMID:15155912 Backbone Marker:Novagen; Backbone Size:0; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin K218T 2026-08-15 01:04:02 0
7093 pNOTAG Hsp104 T317A
 
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RRID:Addgene_1242 Hsp104 (T317A) Saccharomyces cerevisiae Ampicillin PMID:15155912 Backbone Size:6526; Vector Backbone:pNOTAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T317A 2026-08-15 01:04:03 0
7092 pNOTAG Hsp104 R826M
 
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RRID:Addgene_1243 Hsp104 (R826M) Saccharomyces cerevisiae Ampicillin PMID:15155912 Backbone Size:6526; Vector Backbone:pNOTAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin R826M 2026-08-15 01:04:05 0
7090 pNOTAG Hsp104 N728A
 
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RRID:Addgene_1244 Hsp104 (N728A) Saccharomyces cerevisiae Ampicillin PMID:15155912 Backbone Size:6526; Vector Backbone:pNOTAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N728A 2026-08-15 01:04:06 0
6559 pJC25YDJ1 #7
 
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RRID:Addgene_1245 Ydj1 Saccharomyces cerevisiae Ampicillin PMID:9674429 Backbone Size:3654; Vector Backbone:pJC25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 0
pAAV-EF1a-DIO-FLPo-Myc
 
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RRID:Addgene_124641 FLPo Saccharomyces cerevisiae Ampicillin PMID:30773318 Backbone Marker:Karl Deisseroth (pAAV-Ef1a-DIO-hChR2(E123T/T159C)-mCherry, Addgene 35510); Backbone Size:5603; Vector Backbone:pAAV-Ef1a-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 0

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