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Species: Homo sapiens
Genetic Insert: E2F4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7892279
Comments: See author's map.
Proper citation: RRID:Addgene_10914 Copy
Species: Homo sapiens
Genetic Insert: cyclin B2
Vector Backbone Description: Backbone Size:5542; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1388095
Comments: See author's map.
Proper citation: RRID:Addgene_10911 Copy
Species: Synthetic
Genetic Insert: mito-gTEMP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28212432
Proper citation: RRID:Addgene_109117 Copy
Species: Synthetic
Genetic Insert: PTSG_00306 geranyl-geranylation signal
Vector Backbone Description: Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0104 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109096 Copy
Species: Synthetic
Genetic Insert: mTFP1_3'UTR Act
Vector Backbone Description: Vector Backbone:pDSB_0050; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0093 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109097 Copy
Genetic Insert: ChR2-mRuby2-ST
Vector Backbone Description: Backbone Size:5852; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109125 Copy
Species: Mus musculus
Genetic Insert: ChroME(F243Y)-mRuby2-ST
Vector Backbone Description: Backbone Size:5855; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109127 Copy
Genetic Insert: ChroME(S273A)-mRuby2-ST
Vector Backbone Description: Backbone Size:5855; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109128 Copy
Species: Homo sapiens
Genetic Insert: human APOBEC3A
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30295673
Proper citation: RRID:Addgene_109231 Copy
Vector Backbone Description: Backbone Size:7170; Vector Backbone:pEMBL8; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30026278
Proper citation: RRID:Addgene_109320 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Size:5283; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15589161
Proper citation: RRID:Addgene_10921 Copy
Vector Backbone Description: Backbone Size:6967; Vector Backbone:pEMBL8; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30619914
Proper citation: RRID:Addgene_109314 Copy
Species: Rattus norvegicus
Genetic Insert: neuNT
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pSV2 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2871941
Comments: See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Has normal neu cDNA as in pSV2 neuN with transforming sequence (1899-2387) of pSV2 neuT. Expressed in NIH cells at higher levels than pSV2 neuT.
Proper citation: RRID:Addgene_10918 Copy
Species: Rattus norvegicus
Genetic Insert: neuN
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pSV2 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2871941
Comments: See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Expresses better than pSV2 neuT. Has about 100 nt of 5' untranslated region.
Proper citation: RRID:Addgene_10917 Copy
Species: Homo sapiens
Genetic Insert: p16 INK4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7603984
Comments: A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4.
The insert corresponds to isoform 1 but is missing the first 8 amino acids.
Proper citation: RRID:Addgene_10916 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Size:3082; Vector Backbone:pKM464; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating deletion of mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109301 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109233 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109253 Copy
Species: Homo sapiens
Genetic Insert: GαS
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109350 Copy
Species: Homo sapiens
Genetic Insert: Cyclin E1
Vector Backbone Description: Vector Backbone:pInducer20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29148972
Proper citation: RRID:Addgene_109348 Copy
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