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Species: Synthetic
Genetic Insert: mCherry-mVenus
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23654270
Proper citation: RRID:Addgene_42133 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22205962
Proper citation: RRID:Addgene_42081 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22205962
Proper citation: RRID:Addgene_42082 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22205962
Proper citation: RRID:Addgene_42083 Copy
Species: Drosophila melanogaster
Genetic Insert: scribble
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688207
Proper citation: RRID:Addgene_42064 Copy
Genetic Insert: DsRed shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6348; Vector Backbone:pSuper.Retro.Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150874
Proper citation: RRID:Addgene_42053 Copy
Species: Synthetic
Genetic Insert: RT-cassette
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:7904; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition enabling stable propagation of RT-cassette. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42150 Copy
Species: Homo sapiens
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP-TOPO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21668996
Proper citation: RRID:Addgene_42142 Copy
Species: Mus musculus
Genetic Insert: Frizzled 7
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Proper citation: RRID:Addgene_42259 Copy
Genetic Insert: Streptococcus pyogenes Cas9-3X Flag
Vector Backbone Description: Vector Backbone:unknown; Vector Types:CRISPR, zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23360964
Comments: Plasmid Features:
CAG enhancer-CMV promoter = 7466-370
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
BGHpA = 4612-4839
Proper citation: RRID:Addgene_42252 Copy
Species: Mus musculus
Genetic Insert: Frizzled 2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Comments: Fzd2 insert contains S193A compared with NM_020510.2. Mutation has no effect on plasmid function
Proper citation: RRID:Addgene_42254 Copy
Species: Mus musculus
Genetic Insert: Frizzled 4
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Proper citation: RRID:Addgene_42256 Copy
Species: Mus musculus
Genetic Insert: Frizzled 6
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Proper citation: RRID:Addgene_42258 Copy
Vector Backbone Description: Backbone Marker:Joung lab DR274; Backbone Size:2147; Vector Backbone:Zebrafish-gRNA-ExpressionVector; Vector Types:Worm Expression, CRISPR, zebrafish expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23360964
Proper citation: RRID:Addgene_42250 Copy
Genetic Insert: PSD95
Vector Backbone Description: Vector Backbone:pCS6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103964
Proper citation: RRID:Addgene_42240 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22308401
Proper citation: RRID:Addgene_42239 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin 1 S412D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Comments: Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.
Proper citation: RRID:Addgene_42196 Copy
Species: Mus musculus
Genetic Insert: Frizzled 4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12958364
Comments: Fz4-GFP was generated by fusing EGFP (Clontech) at its C-terminus.
Alternate plasmid names: pEGFPN2 mFz4; pEGFP-N2-Frizzled4
Proper citation: RRID:Addgene_42197 Copy
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23287718
Comments: Alternate plasmid name: pSpCas9(BB) (PX330)
For plasmid usage, please see the associated publication (Cong et al. Science. 2013, PMID: 23287718), as well as Ran et al. Nat Protoc. 2013, PMID: 24157548.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_42230 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:QB3 MacroLab; Backbone Size:6145; Vector Backbone:MacroLab 6D; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23386978
Proper citation: RRID:Addgene_42234 Copy
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