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Species: Synthetic
Genetic Insert: ECFP-EYFP FRET positive control
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24108638
Proper citation: RRID:Addgene_109330 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109417 Copy
Vector Backbone Description: Backbone Marker:Paul Odgren (Addgene plasmid # 73582); Backbone Size:8411; Vector Backbone:pLenti-CMV-MCs-SV-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_109377 Copy
Species: Other
Genetic Insert: "capacity monitor" (https://www.addgene.org/66073/) has been inserted in the lambda phage integration site.
Vector Backbone Description: Vector Backbone:NCBI RefSeq NC_010473.1 for DH10B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109392 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109421 Copy
Species: Homo sapiens
Genetic Insert: Rod opsin
Vector Backbone Description: Backbone Size:5374; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23554393
Comments: Also see Ballister et al. BMC Biol. 2018 (doi: 10.1186/s12915-017-0475-2) for additional use.
Proper citation: RRID:Addgene_109361 Copy
Species: Homo sapiens
Genetic Insert: Gsz chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109355 Copy
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:7636; Vector Backbone:pMLM3705 (Addgene plasmid #47754).; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29524148
Comments: The DNA fragment encoding the effector domain is synthesized by PCR and cloned between the AscI and PacI sites of pdCas9-NED to create in-frame fusion between dCas9 (D10A, H840A) and the effector domain. For experiments involving cell lines stably expressing the dCas9-effector domain fusion protein, the coding sequence of the effector domain can be excised from the pdCas9-NED-based recombinant plasmid and transferred into pHAGE EF1α dCas9-NED (Addgene plasmid #109369). Cloning the AscI-PacI fragment between the MluI and AsiSI sites of pHAGE EF1α dCas9-NED preserves the reading frame of the fusion protein (Goubert et al., 2018).
Proper citation: RRID:Addgene_109358 Copy
Species: Homo sapiens
Genetic Insert: Gsi chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109373 Copy
Species: Homo sapiens
Genetic Insert: HDR-Enhancer domain of human CtIP protein
Vector Backbone Description: Backbone Marker:Church lab A; Vector Backbone:hCas9 Addgene ID 41815; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29556040
Comments: Note from depositor: The sequence used for the HDR enhancer domain of CtIP has several differences compared to the reference human CtIP cDNA sequence including T176A. These differences were present in the CtIP expression vector used to make the constructs and do not affect activity.
Proper citation: RRID:Addgene_109400 Copy
Species: Homo sapiens
Genetic Insert: CtIP
Vector Backbone Description: Backbone Marker:Church lab; Vector Backbone:hCas9 Addgene ID 41815; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29556040
Proper citation: RRID:Addgene_109403 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See author's map.
Proper citation: RRID:Addgene_10948 Copy
Species: Danio rerio
Genetic Insert: lamp1
Vector Backbone Description: Vector Backbone:pDEST-actc1b-ccdb-mKate2; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109507 Copy
Species: Homo sapiens
Genetic Insert: MDR1
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pHa; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2898143
Comments: Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines.
Figure legend for author's map:
Structure of retroviral vector carrying MDR] cDNA.
sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho
I. bp, Base pairs; amp, ampicillin resistance gene.
Proper citation: RRID:Addgene_10957 Copy
Species: Homo sapiens
Genetic Insert: Tumor necrosis factor alpha related apoptosis inducing ligand
Vector Backbone Description: Backbone Size:4727; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11309289
Proper citation: RRID:Addgene_10953 Copy
Species: Synthetic
Genetic Insert: mCherry T2A GFP
Vector Backbone Description: Backbone Size:9269; Vector Backbone:HIV-1 NL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29746667
Proper citation: RRID:Addgene_109428 Copy
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET21d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109424 Copy
Species: Homo sapiens
Genetic Insert: Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain
Vector Backbone Description: Backbone Marker:David Liu; Backbone Size:7825; Vector Backbone:BE3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29746667
Proper citation: RRID:Addgene_109426 Copy
Species: Synthetic
Genetic Insert: mCherry T2A GFP
Vector Backbone Description: Backbone Size:9268; Vector Backbone:HIV-1 NL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29746667
Proper citation: RRID:Addgene_109427 Copy
Species: Synthetic
Genetic Insert: actc1b- mKate2-KDEL-pA
Vector Backbone Description: Vector Backbone:actc1b-ccdb; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109494 Copy
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