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Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 23476
Proper citation: RRID:Addgene_166518 Copy
Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 6597
Proper citation: RRID:Addgene_166519 Copy
Species: Homo sapiens
Genetic Insert: GGamma5-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_166777 Copy
Species: Homo sapiens
Genetic Insert: GGamma10-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_166779 Copy
Species: Homo sapiens
Genetic Insert: Noxa
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Venus fused to the N-terminus of human Noxa amino acid sequence: "MPGKKARKNAQPSPARAPAELEVECATQLRRFGDKLNFRQKLLNLISKLFCSGT". Linked by a 30 amino acid (aa) linker.
Proper citation: RRID:Addgene_166762 Copy
Species: Homo sapiens
Genetic Insert: BimEL(Mus musculus)-(Noxa BH3)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore.
Proper citation: RRID:Addgene_166761 Copy
Species: Homo sapiens
Genetic Insert: MAO tail anchor
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: "WERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS" amino acid targeting sequence, which we refer to as "MAO", for mitochondrial outer membrane targeting. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "7aa" indicates the 7 amino acid flexible linker region between Venus and the ActA targeting signal for mitochondria.
Proper citation: RRID:Addgene_166765 Copy
Species: Homo sapiens
Genetic Insert: Angiomotin p130 L129A P130A
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166711 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L W576A
Vector Backbone Description: Backbone Size:5842; Vector Backbone:pCI-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166710 Copy
Species: Homo sapiens
Genetic Insert: AAVR-FLAG
Vector Backbone Description: Backbone Marker:addgene plasmid #17452; Backbone Size:9628; Vector Backbone:pLenti-CMV-Puro-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26814968
Proper citation: RRID:Addgene_166716 Copy
Species: Homo sapiens
Genetic Insert: Angiomotin p130 L106P
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166714 Copy
Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pFastBac HTB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Insect cell expression vector for DPP9S (protein purification)
Proper citation: RRID:Addgene_166787 Copy
Species: Homo sapiens
Genetic Insert: GGamma12-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_166781 Copy
Species: Homo sapiens
Genetic Insert: AMOT PPxY1 fused to NEDD4L WW3
Vector Backbone Description: Backbone Size:5597; Vector Backbone:pCA528; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166705 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L WW2 domain
Vector Backbone Description: Backbone Size:5797; Vector Backbone:pGEX-PP-GFP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166702 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L W221A
Vector Backbone Description: Backbone Size:5842; Vector Backbone:pCI-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166707 Copy
Species: Homo sapiens
Genetic Insert: Bik
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166737 Copy
Species: Homo sapiens
Genetic Insert: Puma
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha.
Proper citation: RRID:Addgene_166739 Copy
Species: Homo sapiens
Genetic Insert: Mcl-1
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins.
Proper citation: RRID:Addgene_166752 Copy
Species: Homo sapiens
Genetic Insert: Bad-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBad-4E" (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166740 Copy
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