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Species: other
Genetic Insert: Phosphinothricin gene regulated by Nos promoter and terminator
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 47999); Vector Backbone:pICH41331; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41918164
Proper citation: RRID:Addgene_232550 Copy
Species: other
Genetic Insert: RbcSE9 terminator
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 51833); Vector Backbone:pAGM9121; Vector Types:Terminator; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41918164
Proper citation: RRID:Addgene_232555 Copy
Species: other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 47988); Vector Backbone:pAGM1299; Vector Types:Coding Sequence; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41918164
Proper citation: RRID:Addgene_232558 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_235330 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_235335 Copy
Species: other
Genetic Insert: VHHGFP4
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 47987); Vector Backbone:pICH41258; Vector Types:Coding Sequence; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41918164
Proper citation: RRID:Addgene_232571 Copy
Species: other
Genetic Insert: ALFA-CENH3
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 48075); Vector Backbone:pICH86966; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41918164
Proper citation: RRID:Addgene_232575 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 4, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249996 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 5, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249997 Copy
Species: Other
Genetic Insert: CDS, lv 0, lux operon
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249912 Copy
Species: Other
Genetic Insert: ABR, lv0, Akan, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249985 Copy
Species: Other
Genetic Insert: dropout part, lv0*, 1R-5O, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250039 Copy
Species: Other
Genetic Insert: dropout part, lv0*, 1C-5C, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250040 Copy
Species: Other
Genetic Insert: dBa1Cas12a2 E1032A C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253636 Copy
Species: Other
Genetic Insert: ca23Cas12a3 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253638 Copy
Species: Other
Genetic Insert: MpCas12a2 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253633 Copy
Species: Other
Genetic Insert: GUS
Vector Backbone Description: Backbone Marker:Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.; Vector Backbone:pJRT.Agro.CLCrVA.008; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40394900
Proper citation: RRID:Addgene_234916 Copy
Species: Other
Genetic Insert: mGreenLantern
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40932770
Proper citation: RRID:Addgene_246580 Copy
Species: Other
Genetic Insert: MapTauC-mScarlet3-H
Vector Backbone Description: Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_242974 Copy
Species: Other
Genetic Insert: RBS S3
Vector Backbone Description: Vector Backbone:pCloneEZ-NRS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41699564
Comments: Note: Some sequence editors do not support enzymatic digestion and ligation reactions with both sense and antisense parts. Accordingly, the reverse compliment of this sequence’s sequencing results can be used, with the other parts in the CyanoConstruct part library, for in silico proofing.
Proper citation: RRID:Addgene_251388 Copy
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