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Species: Silene conica
Genetic Insert: N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito
Vector Backbone Description: Vector Backbone:pK7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37463427
Proper citation: RRID:Addgene_202659 Copy
Species: Silene conica
Genetic Insert: N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid
Vector Backbone Description: Vector Backbone:pK7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37463427
Proper citation: RRID:Addgene_202660 Copy
Species: Arabidopsis thaliana
Genetic Insert: temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet ; Backbone Size:2073; Vector Backbone:pAGM3946; Vector Types:CRISPR, Synthetic Biology, MoClo compatible Level 0 module ; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36382936
Proper citation: RRID:Addgene_192453 Copy
Species: Synthetic
Genetic Insert: GvpN-Linker-Large BiT
Vector Backbone Description: Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39227754
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.23.550228v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216963 Copy
Species: E. coli
Genetic Insert: tnsABCD
Vector Backbone Description: Vector Backbone:pIND4; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39324814
Comments: Needs to be grown in E. coli BW25141 (pir+ strain with an anti-CRISPR protein on the chromosome)
Please visit https://pubmed.ncbi.nlm.nih.gov/37662258/ for bioRxiv preprint.
Proper citation: RRID:Addgene_220900 Copy
Species: N. nambi
Genetic Insert: CPH
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215247 Copy
Species: Synthetic
Genetic Insert: CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215261 Copy
Species: Synthetic
Genetic Insert: PDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215259 Copy
Species: Synthetic
Genetic Insert: pMAPKKK18:HISPS.TNOS-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215272 Copy
Species: Synthetic
Genetic Insert: CBS-att-OCS-HispS-tNos-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215268 Copy
Species: Escherichia coli K-12 MG1655
Genetic Insert: yhaO
Vector Backbone Description: Vector Backbone:pTR48; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37980564
Proper citation: RRID:Addgene_207654 Copy
Species: Synthetic
Genetic Insert: P35S:HISPS-SF-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215274 Copy
Species: Synthetic
Genetic Insert: PNOS:HISPS-SF-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215273 Copy
Species: Arabidopsis thaliana
Genetic Insert: GRF4 isoform 1
Vector Backbone Description: Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39100877
Comments: GRF4 - Transcript isoform AT1G35160.1 is used in this plasmid.
Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_216140 Copy
Species: Arabidopsis thaliana
Genetic Insert: Guide RNA against AtAGAMOUS1
Vector Backbone Description: Vector Backbone:35s:RUBY and pRU53; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_225983 Copy
Species: Other
Genetic Insert: mCherry-UnaG
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38466577
Proper citation: RRID:Addgene_221660 Copy
Species: Other
Genetic Insert: AePHOT
Vector Backbone Description: Vector Backbone:pMpGWB306; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882095
Proper citation: RRID:Addgene_220111 Copy
Genetic Insert: EtA3C-zCas9(D10A)-UGI
Vector Backbone Description: Vector Backbone:pYPQ255; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39950393
Proper citation: RRID:Addgene_225146 Copy
Genetic Insert: DlA3G-zCas9(D10A)-UGI
Vector Backbone Description: Vector Backbone:pYPQ255; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39950393
Proper citation: RRID:Addgene_225148 Copy
Species: Bacteriophage M13
Genetic Insert: gp1-gp11
Vector Backbone Description: Vector Backbone:pSEVA471; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Please also refer to Pujar et al. 2024 Nucleic Acids Res. (https://doi.org/10.1093/nar/gkae1256, and see https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint) for additional work using this plasmid.
Proper citation: RRID:Addgene_231420 Copy
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