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Species: Mus musculus
Genetic Insert: Frizzled 8
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Proper citation: RRID:Addgene_42260 Copy
Species: Mus musculus
Genetic Insert: Frizzled 9
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Proper citation: RRID:Addgene_42261 Copy
Species: Homo sapiens
Genetic Insert: PTK2BA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3CC6/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42401 Copy
Species: Homo sapiens
Genetic Insert: NUDT3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2FVV/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42348 Copy
Species: Homo sapiens
Genetic Insert: p18
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42338 Copy
Species: Homo sapiens
Genetic Insert: p14
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42330 Copy
Species: Homo sapiens
Genetic Insert: p18
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42331 Copy
Genetic Insert: humanized S. pyogenes Cas9 (D10A) nickase
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23287718
Comments: Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_42333 Copy
Genetic Insert: humanized S. pyogenes Cas9 (D10A) nickase
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23287718
Comments: Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_42335 Copy
Species: Homo sapiens
Genetic Insert: p18 G2A
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42327 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Prof. Amir Sharon (Tel Aviv University, Israel); Vector Backbone:pSH1; Vector Types:Filamentous Ascomycetes EGFP expression; Bacterial Resistance:Ampicillin
Comments: Plasmid provided by Prof. Amir Sharon (Tel Aviv University, Israel)
The full plasmid sequence is assembled and not the result of sequencing, there are some discrepancies between the Addgene QC sequence and the full plasmid sequence. These differences are in the plasmid backbone regions.
Proper citation: RRID:Addgene_42323 Copy
Species: Homo sapiens
Genetic Insert: VAMP 3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9614185
Comments: Full-length cDNA of the human isoform of cellubrevin, was obtained by reverse transcription-PCR on CaCo-2 mRNA using standard procedures and the following set of oligonucleotides:
5'-ATGTCTACAGGTCCAACTGCTG-3' (hcb5')
and
5'-GCTGGTTCTTCATGAAGAGACA-3' (hcb3')
Proper citation: RRID:Addgene_42310 Copy
Species: Homo sapiens
Genetic Insert: PARP15A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3BLJ/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42398 Copy
Species: Homo sapiens
Genetic Insert: VAMP 4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11839770
Proper citation: RRID:Addgene_42313 Copy
Species: Homo sapiens
Genetic Insert: STARD5A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2R55/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42392 Copy
Genetic Insert: VAMP 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12853575
Comments: The V8A & T116S substitutions found in the Addgene sequence correspond to polymorphisms and have no functional impact.
Proper citation: RRID:Addgene_42308 Copy
Species: E. coli O157:H7
Genetic Insert: ChuA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3418; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21171606
Proper citation: RRID:Addgene_42539 Copy
Species: Arabidopsis thaliana
Genetic Insert: FKF1 (N-LOV, G128D mutant) fused with VP16
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801976
Proper citation: RRID:Addgene_42499 Copy
Species: Homo sapiens
Genetic Insert: shRNA-PKM2
Vector Backbone Description: Backbone Marker:The RNAi consortium; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect.
Proper citation: RRID:Addgene_42516 Copy
Species: Arabidopsis thaliana
Genetic Insert: GIGANTEA fused with Gal4 DBD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801976
Proper citation: RRID:Addgene_42500 Copy
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