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Species: Other
Genetic Insert: RBS S2
Vector Backbone Description: Vector Backbone:pEZclone-NRS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41699564
Proper citation: RRID:Addgene_251387 Copy
Species: Other
Genetic Insert: Promoter A1lacO-1
Vector Backbone Description: Vector Backbone:pCloneEZ-NRS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41699564
Proper citation: RRID:Addgene_251365 Copy
Species: Other
Genetic Insert: RBS S2
Vector Backbone Description: Vector Backbone:pEZclone-NRS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41699564
Proper citation: RRID:Addgene_251377 Copy
Species: Other
Genetic Insert: J23119 CAO1 target + GAPDH T17 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253628 Copy
Species: Other
Genetic Insert: J23119 GAPDH T17 target + GAPDH T17 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253629 Copy
Species: Other
Genetic Insert: J23119 GAPDH T17 target + CAO1 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253630 Copy
Species: Other
Genetic Insert: PxylA
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235411 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone: pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235362 Copy
Species: Other
Genetic Insert: Bba_B0015
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235369 Copy
Species: Other
Genetic Insert: S718 term
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235439 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235443 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235445 Copy
Species: Other
Genetic Insert: Olyzias latipes Tol1 transposase
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_253476 Copy
Species: Other
Genetic Insert: inducible promoter, lv0, PTet_MC
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249884 Copy
Species: Other
Genetic Insert: inducible promoter, lv0, PBDA
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249885 Copy
Species: Other
Genetic Insert: CDS, lv 0, sfGfp
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249924 Copy
Species: Other
Genetic Insert: CDS, lv 0, VioC
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249921 Copy
Species: Other
Genetic Insert: CDS, lv 0, VioB
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249920 Copy
Species: Other
Genetic Insert: CDS, lv 0, VioE
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249922 Copy
Species: Other
Genetic Insert: inducible promoter, lv0, PTac
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249891 Copy
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