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Species: Mus musculus
Genetic Insert: sgRNA targeting mouse CD90.2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29445007
Comments: Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82670 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting mouse CD45.2 region 1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29445007
Comments: Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82672 Copy
Species: Mus musculus
Genetic Insert: 1kb genomic DNA sequence from murine wildtype Foxp3
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29445007
Comments: This HDR template can be used in conjunction with sgRNA Foxp3K276X (Addgene ID 82675) or sgRNA Foxp3sf/J (Addgene ID 82676). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82664 Copy
Species: Mus musculus
Genetic Insert: 2kb genomic DNA sequence from murine wildtype Foxp3
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29445007
Comments: This HDR template can be used in conjunction with sgRNA Foxp3K276X (Addgene ID 82675) or sgRNA Foxp3sf/J (Addgene ID 82676). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82665 Copy
Species: Mus musculus
Genetic Insert: 160bp genomic DNA sequence from murine CD90.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29445007
Comments: This HDR template can be used in conjunction with sgRNA CD90.2 (Addgene ID 82670 or Addgene ID 82671). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82666 Copy
Species: Mus musculus
Genetic Insert: mouse NFL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4242; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17285620
Comments: Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.
Mouse NFL cDNA sequence deposited in Genbank (Accession number DQ201635).
Proper citation: RRID:Addgene_83127 Copy
Species: Mus musculus
Genetic Insert: mouse NFM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4047; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17285620
Comments: Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.
Mouse NFM cDNA sequence deposited in Genbank (Accession number DQ201636).
Proper citation: RRID:Addgene_83126 Copy
Species: Mus musculus
Genetic Insert: shId2
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the TRC ( TRCN0000229535). Knockdown validated by Western blot of transient, ectopic expression of murine Id2 in 293T cells.
Proper citation: RRID:Addgene_83088 Copy
Species: Mus musculus
Genetic Insert: shNKCC1
Vector Backbone Description: Backbone Marker:Jacks Lab (MIT); Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23015452
Comments: This plasmid contains a c4351t substitution within the shRNA. The depositing lab states that they do not know whether this mutation affects plasmid function.
pSico allows for conditional (Cre-Lox), stable expression of shNKCC1. Cre expression excises GFP and allows for shNKCC1 expression.
Proper citation: RRID:Addgene_83027 Copy
Species: Mus musculus
Genetic Insert: CAPZB2
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83194 Copy
Species: Mus musculus
Genetic Insert: CAPZA1
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83193 Copy
Species: Mus musculus
Genetic Insert: GATA binding protein 3
Vector Backbone Description: Backbone Size:12400; Vector Backbone:Lazarus (LZRS); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80135 Copy
Species: Mus musculus
Genetic Insert: Runx1
Vector Backbone Description: Backbone Marker:Dr. John Rossi, Beckman Research Institute of the City of Hope, CA; Vector Backbone:Banshee; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80157 Copy
Species: Mus musculus
Genetic Insert: PU.1
Vector Backbone Description: Backbone Marker:Rothernberg lab, Addgene plasmid 80139; Vector Backbone:IRES-mCherry; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23868921
Proper citation: RRID:Addgene_80141 Copy
Species: Mus musculus
Genetic Insert: PU.1
Vector Backbone Description: Backbone Marker:Rothernberg lab, Addgene plasmid 80139; Vector Backbone:IRES-mCherry; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23868921
Proper citation: RRID:Addgene_80140 Copy
Species: Mus musculus
Genetic Insert: OKMS
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25772473
Comments: Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.
Proper citation: RRID:Addgene_80480 Copy
Species: Mus musculus
Genetic Insert: WTSI
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25772473
Comments: Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.
Proper citation: RRID:Addgene_80486 Copy
Species: Mus musculus
Genetic Insert: lin-28 homolog A (C. elegans)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25772473
Comments: Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.
Proper citation: RRID:Addgene_80485 Copy
Species: Mus musculus
Genetic Insert: Smad7
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80896 Copy
Species: Mus musculus
Genetic Insert: Smad7
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80895 Copy
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