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Species: Mus musculus
Genetic Insert: RASL10B
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55673 Copy
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12623879
Comments: The distance between the two Not1 sites is ~6kb
Clone into the Sal1 site (or Sal/Hind3 site).
Release the pBluescript backbone with Not1 for transgenics.
This construct consists of a full-length α-MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.
Proper citation: RRID:Addgene_55792 Copy
Species: Mus musculus
Genetic Insert: p110 beta
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55719 Copy
Species: Mus musculus
Genetic Insert: p110 alpha
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55713 Copy
Species: Mus musculus
Genetic Insert: GEM
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55680 Copy
Species: Mus musculus
Genetic Insert: p110 alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Comments: murine p110α and p110β constructs (wild type and RBD DM) subcloned into pBacPAK-His3-GST (from protein purification facility). So these constructs encode GST-tagged p110, which can be cleaved of using rhinovirus 3C protease
Proper citation: RRID:Addgene_55722 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens protein kinase C, iota (PRKCI)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18270268
Proper citation: RRID:Addgene_55687 Copy
Species: Mus musculus
Genetic Insert: RASL11A
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55685 Copy
Species: Homo sapiens
Genetic Insert: Very long-chain specific acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19889959
Proper citation: RRID:Addgene_55823 Copy
Species: Rattus norvegicus
Genetic Insert: alpha-s (alpha3beta5/G226A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11927592
Proper citation: RRID:Addgene_55798 Copy
Species: Mus musculus
Genetic Insert: Tim1 (BL/6 allele)
Vector Backbone Description: Backbone Marker:Kane Lab; Vector Backbone:pCDEF3-CD8SS-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16284246
Proper citation: RRID:Addgene_49205 Copy
Species: Bos taurus
Genetic Insert: H6-FGB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24068983
Comments: http://www.pnas.org/content/105/1/365.long
Proper citation: RRID:Addgene_49203 Copy
Species: Mus musculus
Genetic Insert: Tim3 (del.Cyto)
Vector Backbone Description: Vector Backbone:pCDEF3-CD8SS-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807895
Proper citation: RRID:Addgene_49209 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49285 Copy
Species: Homo sapiens
Genetic Insert: GABA(A) receptor subunit alpha-2
Vector Backbone Description: Backbone Marker:BD PharMingen ; Backbone Size:5084; Vector Backbone:modified pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18256255
Proper citation: RRID:Addgene_49169 Copy
Species: Bos taurus
Genetic Insert: FlincG3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24068983
Comments: http://www.pnas.org/content/105/1/365.long
Proper citation: RRID:Addgene_49202 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49281 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49280 Copy
Species: Homo sapiens
Genetic Insert: Eya2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1(+) Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49264 Copy
Species: Homo sapiens
Genetic Insert: Six1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV 2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49263 Copy
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