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Species: Saccharomyces cerevisiae
Genetic Insert: Pgal1TRP1URA3
Vector Backbone Description: Backbone Size:7374; Vector Backbone:pYC6-CT/LYS2 hybrid; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.
Proper citation: RRID:Addgene_11010 Copy
Genetic Insert: mCherry-GFP-LC3
Vector Backbone Description: Vector Backbone:FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590078
Proper citation: RRID:Addgene_110060 Copy
Species: Homo sapiens
Genetic Insert: eEF2K (eukaryotic elongation factor 2 kinase)
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22669845
Proper citation: RRID:Addgene_110160 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29779950
Proper citation: RRID:Addgene_110135 Copy
Species: Homo sapiens
Genetic Insert: EGFR D770-N771 insNPG
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16187797
Comments: Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Mutation made by using the following primers to PCR from plasmid 11011: 5'-GGCCAGCGTGGACAACCCCGGCAACCCCCACGT-3' and 5'-ACGTGGGGGTTGCCGGGGTTGTCCACGCTGGCC-3'
Proper citation: RRID:Addgene_11016 Copy
Species: Homo sapiens
Genetic Insert: EGFR (del3)
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16187797
Comments: Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Mutation made by using the following primers to PCR from plasmid 11011: 5'-GTCGCTATCAAGGAACCAACATCTCCGAAA-3' and 5'-TTTCGGAGATGTTGGTTCCTTGATAGCGAC-3'
Proper citation: RRID:Addgene_11015 Copy
Species: Homo sapiens
Genetic Insert: EGFR D837A
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16187797
Comments: Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-GGTGCACCGCGCCCTGGCAGCCA-3' and 5'-TGGCTGCCAGGGCGCGGTGCACC-3'
Proper citation: RRID:Addgene_11014 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110110 Copy
Species: Mus musculus
Genetic Insert: MeCP2_e2 FL [R111G] (mouse cDNA)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4680; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29019980
Proper citation: RRID:Addgene_110187 Copy
Species: Other
Genetic Insert: scaffold Sybody of the convex library
Vector Backbone Description: Vector Backbone:pBXPHM3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29792401
Proper citation: RRID:Addgene_110104 Copy
Species: A. victoria
Genetic Insert: eGFPemerald
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:2700; Vector Backbone:pUC18; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11771662
Comments: GFPemerald purchased from Packard.
Proper citation: RRID:Addgene_110210 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25261934
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110215 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25261934
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110214 Copy
Species: Mus musculus
Genetic Insert: SRY (sex determining region Y)-box 2
Vector Backbone Description: Backbone Size:9501; Vector Backbone:pCWtre-hpaI-pacI-rtTAiBlast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30332632
Proper citation: RRID:Addgene_110280 Copy
Species: Homo sapiens
Genetic Insert: Elongin BC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.
Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.
Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...
2C9W: https://www.thesgc.org/structures/2C9W.
Proper citation: RRID:Addgene_110274 Copy
Species: Mus musculus
Genetic Insert: chemokine (C-X-C motif) ligand 5
Vector Backbone Description: Backbone Size:11000; Vector Backbone:GFP.Cre empty vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30332632
Proper citation: RRID:Addgene_110278 Copy
Species: Mus musculus
Genetic Insert: Tcf-4
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:Mammalian Expression, Xenopus, Avian, and Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9880534
Comments: Positive clone encoding mTcf4 obtained from screening a mouse embryonic gut cDNA library with degenerate oligos complementary to the highly conserved HMG box of Tcf/LEF proteins.
Proper citation: RRID:Addgene_11031 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: cDNA encoded from 659-4371 (see author's map).
Proper citation: RRID:Addgene_11027 Copy
Species: Mus musculus
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: No stop codon.
Proper citation: RRID:Addgene_11026 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9032279
Proper citation: RRID:Addgene_11025 Copy
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