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Species: Saccharomyces cerevisiae
Genetic Insert: Gal80(opt)
Vector Backbone Description: Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21905164
Comments: pME-NLS-Gal80opt was generated by PCR using primers 5'-ATGGCTCCAAAGAAGAAGCGTAAGGTAATGGACTACAACAAAAGGAGCAG-3' for the nuclear localization signal (Kwan et al., 2007) and 5'-CAGTGAGTAGTGAGAGATATTTG-3' for codon-optimized Gal80 (GenScript; GenBank accession no. JN314417).
Proper citation: RRID:Addgene_51618 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal80ts
Vector Backbone Description: Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21905164
Comments: pME-Gal80ts was cloned from pBPHLWL-Gal80ts (McGuire et al., 2003) by PCR (kind gift of T. Clandinin).
*Also has a non-functional E101D mutation
Proper citation: RRID:Addgene_51619 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747889
Proper citation: RRID:Addgene_51627 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24747889
Proper citation: RRID:Addgene_51626 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal80
Vector Backbone Description: Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21905164
Proper citation: RRID:Addgene_51621 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:YDpL; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M3939 (leu2::ADE2) was made by cutting YDp-L with EcoRV, and inserting a 3.6 kb BamHI (blunted with Klenow) fragment with ADE2 containing plasmid M1144.
Proper citation: RRID:Addgene_51672 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:YDpW; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M3938 (trp1::ADE2 ) was made by cutting YDp-W with EcoRV and inserting a 3.6 kb BamHI (blunted with Klenow) fragment containing ADE2 from plasmid M1144.
Proper citation: RRID:Addgene_51673 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:YDp-K; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558.
Proper citation: RRID:Addgene_51675 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LYS2
Vector Backbone Description: Vector Backbone:pIC19H–SXL2; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H–SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771.
Proper citation: RRID:Addgene_51677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid # 51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4753 (kanMX::ADE2) was made by digesting M4757 with KpnI and SalI and inserting a 2.3 kb KpnI–SalI fragment with ADE2 from plasmid pFA6–ADE2.
Proper citation: RRID:Addgene_51683 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4754 (kanMX::HIS3) was made by digesting M4757 with XmaI and SalI and inserting a 1.2 kb XmaI–SalI fragment with HIS3 from plasmid YDp-H.
Proper citation: RRID:Addgene_51684 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Vector Backbone:pAG25 (Goldstein and McCusker, 1999); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: First, plasmid pAG25 (Goldstein and McCusker, 1999) was digested with HindIII and PacI, treated with Klenow and ligated closed. This plasmid was digested with KpnI and SphI and the 0.9 kb KpnI–SphI fragment containing TRP1 from pJJ246 was inserted, constructing M4757.
Proper citation: RRID:Addgene_51687 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4758 (kanMX::URA3) was made by digesting M4757 with BamHI and SalI and inserting a 1.2 kb BamHI–XhoI fragment with URA3 from plasmid M522.
Proper citation: RRID:Addgene_51688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hisG-URA3-hisG
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI–SalI fragment with hisG–URA3–hisG from plasmid M2524.
The kanMX::hisG–URA3–hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers.
Proper citation: RRID:Addgene_51690 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HST2
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51745 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sir2
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51744 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sir2
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51743 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mKate2
Vector Backbone Description: Backbone Size:5064; Vector Backbone:pST39; Vector Types:Bacterial Expression, p1 Integration Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24487693
Proper citation: RRID:Addgene_52345 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Recoded TP-DNAP
Vector Backbone Description: Backbone Size:6105; Vector Backbone:CEN6-ARS4/ColE1 Yeast/Bacteria Shuttle Vector; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24487693
Proper citation: RRID:Addgene_52346 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: leu*
Vector Backbone Description: Backbone Size:5839; Vector Backbone:pST39; Vector Types:Bacterial Expression, p1 Integration Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24487693
Proper citation: RRID:Addgene_52347 Copy
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