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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA3-Smad7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_80893 | Smad7 | Mus musculus | Ampicillin | Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa 2-426 | 2026-08-15 01:20:42 | 2 | ||
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pcDNA3-ALK6 K231R Resource Report Resource Website |
RRID:Addgene_80884 | ALK6 | Mus musculus | Ampicillin | PMID:26701726 | Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K231R (kinase-deficient) | 2026-08-15 01:20:42 | 0 | |
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pcDNA3-ALK6 wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_80882 | ALK6 | Mus musculus | Ampicillin | PMID:26701726 | Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:42 | 3 | ||
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Flag-Gsdmd Resource Report Resource Website 1+ mentions |
RRID:Addgene_80950 | Gsdmd | Mus musculus | Ampicillin | PMID:27383986 | Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:42 | 6 | ||
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pACYC-mTET1-N Resource Report Resource Website |
RRID:Addgene_81056 | TET1 | Mus musculus | Chloramphenicol | PMID:26932196 | Backbone Marker:Novagen; Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | N-terminus, amino acids 301-1366 | 2026-08-15 01:20:43 | 0 | |
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pCDF-His-mTET1CD Resource Report Resource Website 1+ mentions |
RRID:Addgene_81053 | TET1 | Mus musculus | Streptomycin | PMID:26932196 | Backbone Marker:Novagen; Backbone Size:3780; Vector Backbone:pCDF-Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | catalytic domain amino acid 1367-2057 | 2026-08-15 01:20:43 | 1 | |
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pCDF-His-mTET1CD∆cat Resource Report Resource Website 1+ mentions |
RRID:Addgene_81054 | TET1 | Mus musculus | Streptomycin | PMID:26932196 | Backbone Marker:Novagen; Backbone Size:3780; Vector Backbone:pCDF-Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | catalytic domain amino acid 1367-2057 with histidine 1652 and aspartic acid 1654 changed to tyrosine and alanine, respectively | 2026-08-15 01:20:43 | 1 | |
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pSico-shRaptor Resource Report Resource Website |
RRID:Addgene_81086 | Raptor shRNA | Mus musculus | Ampicillin | PMID:24139800 | pSico allows for conditional (Cre-Lox), stable expression of shRaptor. Addition of Cre excises GFP and allows for shRaptor expression. | Backbone Marker:Jacks Lab; Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:44 | 0 | |
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mPiezo2-CMV-Sport6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_81073 | Piezo2 (please see depositor comments below) | Mus musculus | Ampicillin | PMID:26008989 | Please note- when compared to the Piezo2 reference sequence NM_001039485.4, the insert in this plasmid contains mutation D1896E and deletion of residues V1897 and E1898. Piezo2 was amplified out of mouse DRGs. The variant that is present in DRGs when it was cloned is missing 6 base pairs. Here is the link to the original manuscript—the reported protein sequence is in the supplementary data. The plasmid functions as described. http://science.sciencemag.org/content/330/6000/55.full The depositor recommends the following protocol for researchers having issues growing or isolating plasmid 81073 (mPiezo2-CMV-Sport6): For Piezo2- inoculate culture from glycerol stock into 4-5mls of LB media. Grow the culture at 37 deg for 4-5 hrs and then transfer the culture to larger volumes of media (about 100-150mls) and grow overnight at 37 deg. Usual maxi prep yields are low in the range of 50-100ng/ul. We digest verify the preps by digesting with KpnI and NotI to give 8.2kb insert and 4.4kb vector bands or with KpnI and SpeI to give 2 bands with sizes 8.6 kb and 4.2 kb. When handling glycerol stocks for Piezo2, always re-streak and pick single clones. Never inoculate the glycerol stock into an overnight culture. Our lab has had issues with clones that grow with amp resistance but after digestion, show a prominent ~4 kb single band. After mini-prep or maxi-prep, always confirm the DNA product by restriction digest. We digest with KpnI and SpeI and this leads to 2 bands with sizes 8.6 kb and 4.2 kb. We digest verify every time we maxi prep. For our maxi preps, we notice that our yields are low - sometimes ~ 50-100 ng/ul when eluting in 1 ml from ~150 ml of culture. Alternatively, transform your DNA into XL-10 gold cells. Plate and grow for 3-4 days at 25C. Select single colonies and inoculate 200-300 mL of media. Grow with shaking at 25C for 3-4 days. Maxiprep the culture as normal, digest DNA to verify as above. | Vector Backbone:pCMV-Sport6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:43 | 7 | |
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CRT-GFP pcDNA3.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_80978 | CRT | Mus musculus | Ampicillin | PMID:21785162 | Backbone Marker:Steyger lab; Vector Backbone:GFP-pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | no KDEL | 2026-08-15 01:20:43 | 1 | |
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pTG-mTDGa.0 Resource Report Resource Website |
RRID:Addgene_81048 | TDG | Mus musculus | Ampicillin | PMID:26932196 | Backbone Marker:New England Biolabs; Backbone Size:6550; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:43 | 0 | ||
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pTG-mTDGa.1 Resource Report Resource Website |
RRID:Addgene_81049 | TDG | Mus musculus | Ampicillin | PMID:26932196 | Backbone Marker:New England Biolabs; Backbone Size:6550; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | asparagine 151 changed to alanine | 2026-08-15 01:20:44 | 0 | |
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pCAGGS-4EBP1 F113A Resource Report Resource Website 1+ mentions |
RRID:Addgene_81122 | 4EBP1 F113A | Mus musculus | Ampicillin | PMID:24139800 | These plasmids were also used in the following publication "Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335." | Backbone Size:4802; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | F113A mutation | 2026-08-15 01:20:44 | 2 |
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pSicoR-sh4EBP2 Resource Report Resource Website |
RRID:Addgene_81121 | 4E-BP2 shRNA | Mus musculus | Ampicillin | PMID:24139800 | pSicoR allows for expression of the 4E-BP2 shRNA in the absence of Cre. Expression of Cre turns off shRNA expression. These plasmids were also used in the following publication "Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335." | Backbone Marker:Jacks Lab (MIT); Backbone Size:7567; Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:44 | 0 | |
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pGL2-Skp2 promoter-luciferase Resource Report Resource Website 1+ mentions |
RRID:Addgene_81119 | SKP2 | Mus musculus | Ampicillin | PMID:27309807 | Backbone Marker:Promega; Vector Backbone:pGL2-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | contains the region from 1_kb upstream of transcription start site to 200_bp downstream | 2026-08-15 01:20:44 | 1 | |
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pFUSEss-CHIg-mM_M18 Resource Report Resource Website |
RRID:Addgene_91729 | immunoglobulin heavy constant mu | Mus musculus | Bleocin (Zeocin) | PMID:29323348 | Backbone Marker:Invivogen; Backbone Size:4852; Vector Backbone:pFUSEss-CHIg-mM; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | 2026-08-15 01:22:23 | 0 | ||
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pFUSEss-CHIg-mM-Asn209Leu_M18 Resource Report Resource Website |
RRID:Addgene_91747 | immunoglobulin heavy constant mu | Mus musculus | Bleocin (Zeocin) | PMID:29323348 | Backbone Marker:Invivogen; Backbone Size:4852; Vector Backbone:pFUSEss-CHIg-mM; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | Asn209 changed to Leu (EU numbering) | 2026-08-15 01:22:23 | 0 | |
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pFUSEss-CHIg-mM-Asn209Lys_M18 Resource Report Resource Website |
RRID:Addgene_91748 | immunoglobulin heavy constant mu | Mus musculus | Bleocin (Zeocin) | PMID:29323348 | Backbone Marker:Invivogen; Backbone Size:4852; Vector Backbone:pFUSEss-CHIg-mM; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | Asn209 changed to Lys (EU numbering) | 2026-08-15 01:22:23 | 0 | |
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pFUSEss-CHIg-mM-Asn209His_M18 Resource Report Resource Website |
RRID:Addgene_91745 | immunoglobulin heavy constant mu | Mus musculus | Bleocin (Zeocin) | PMID:29323348 | Backbone Marker:Invivogen; Backbone Size:4852; Vector Backbone:pFUSEss-CHIg-mM; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | Asn209 changed to His (EU numbering) | 2026-08-15 01:22:23 | 0 | |
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pFUSEss-CHIg-mM-Asn209Ile_M18 Resource Report Resource Website |
RRID:Addgene_91746 | immunoglobulin heavy constant mu | Mus musculus | Bleocin (Zeocin) | PMID:29323348 | Backbone Marker:Invivogen; Backbone Size:4852; Vector Backbone:pFUSEss-CHIg-mM; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | Asn209 changed to Ile (EU numbering) | 2026-08-15 01:22:23 | 0 |
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