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pME-nlsGal80(opt)
 
Resource Report
Resource Website 		RRID:Addgene_51618 Gal80(opt) Saccharomyces cerevisiae Kanamycin PMID:21905164 pME-NLS-Gal80opt was generated by PCR using primers 5'-ATGGCTCCAAAGAAGAAGCGTAAGGTAATGGACTACAACAAAAGGAGCAG-3' for the nuclear localization signal (Kwan et al., 2007) and 5'-CAGTGAGTAGTGAGAGATATTTG-3' for codon-optimized Gal80 (GenScript; GenBank accession no. JN314417). Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin codon optimized 2026-08-15 01:16:15 0
pME-Gal80ts
 
Resource Report
Resource Website 		RRID:Addgene_51619 Gal80ts Saccharomyces cerevisiae Kanamycin PMID:21905164 pME-Gal80ts was cloned from pBPHLWL-Gal80ts (McGuire et al., 2003) by PCR (kind gift of T. Clandinin). *Also has a non-functional E101D mutation Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin temperature sensitive (G203V, G323R)* 2026-08-15 01:16:19 0
pREDTAI
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_51627 I-SceI Saccharomyces cerevisiae Ampicillin PMID:24747889 Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Ampicillin 2026-08-15 01:16:15 1
pREDKI
 
Resource Report
Resource Website 		RRID:Addgene_51626 I-SceI Saccharomyces cerevisiae Kanamycin PMID:24747889 Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Kanamycin 2026-08-15 01:16:19 0
pME-basGal80
 
Resource Report
Resource Website 		RRID:Addgene_51621 Gal80 Saccharomyces cerevisiae Kanamycin PMID:21905164 Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:15 0
M3939 leu2::ADE2 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51672 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid M3939 (leu2::ADE2) was made by cutting YDp-L with EcoRV, and inserting a 3.6 kb BamHI (blunted with Klenow) fragment with ADE2 containing plasmid M1144. Vector Backbone:YDpL; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
M3938 trp1::ADE2 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51673 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid M3938 (trp1::ADE2 ) was made by cutting YDp-W with EcoRV and inserting a 3.6 kb BamHI (blunted with Klenow) fragment containing ADE2 from plasmid M1144. Vector Backbone:YDpW; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
D588 lys2::HIS3 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51675 HIS3 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558. Vector Backbone:YDp-K; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
D771 leu2::LYS2 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51677 LYS2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H–SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771. Vector Backbone:pIC19H–SXL2; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M4753 KanMX::ADE2 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51683 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 M4753 (kanMX::ADE2) was made by digesting M4757 with KpnI and SalI and inserting a 2.3 kb KpnI–SalI fragment with ADE2 from plasmid pFA6–ADE2. Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid # 51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M4754 KanMX::HIS3 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51684 HIS3 Saccharomyces cerevisiae Ampicillin PMID:12898713 M4754 (kanMX::HIS3) was made by digesting M4757 with XmaI and SalI and inserting a 1.2 kb XmaI–SalI fragment with HIS3 from plasmid YDp-H. Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
M4757 KanMX::TRP1 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51687 TRP1 Saccharomyces cerevisiae Ampicillin PMID:12898713 First, plasmid pAG25 (Goldstein and McCusker, 1999) was digested with HindIII and PacI, treated with Klenow and ligated closed. This plasmid was digested with KpnI and SphI and the 0.9 kb KpnI–SphI fragment containing TRP1 from pJJ246 was inserted, constructing M4757. Vector Backbone:pAG25 (Goldstein and McCusker, 1999); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
M4758 KanMX::URA3 Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51688 URA3 Saccharomyces cerevisiae Ampicillin PMID:12898713 M4758 (kanMX::URA3) was made by digesting M4757 with BamHI and SalI and inserting a 1.2 kb BamHI–XhoI fragment with URA3 from plasmid M522. Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
 
Resource Report
Resource Website 		RRID:Addgene_51690 hisG-URA3-hisG Saccharomyces cerevisiae Ampicillin PMID:12898713 M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI–SalI fragment with hisG–URA3–hisG from plasmid M2524. The kanMX::hisG–URA3–hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers. Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
p413TEF-HST2
 
Resource Report
Resource Website 		RRID:Addgene_51745 HST2 Saccharomyces cerevisiae Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:21 0
p413TEF-Sir2-P394L
 
Resource Report
Resource Website 		RRID:Addgene_51744 Sir2 Saccharomyces cerevisiae Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin P394L 2026-08-15 01:16:17 0
p413TEF-Sir2-H364Y
 
Resource Report
Resource Website 		RRID:Addgene_51743 Sir2 Saccharomyces cerevisiae Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin H364Y 2026-08-15 01:16:17 0
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
 
Resource Report
Resource Website 		RRID:Addgene_52345 mKate2 Saccharomyces cerevisiae Ampicillin PMID:24487693 Backbone Size:5064; Vector Backbone:pST39; Vector Types:Bacterial Expression, p1 Integration Vector; Bacterial Resistance:Ampicillin changed V2H 2026-08-15 01:16:27 0
pCCL-ReDNAP (Y427A)
 
Resource Report
Resource Website 		RRID:Addgene_52346 Recoded TP-DNAP Saccharomyces cerevisiae Kanamycin PMID:24487693 Backbone Size:6105; Vector Backbone:CEN6-ARS4/ColE1 Yeast/Bacteria Shuttle Vector; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:22 0
pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA
 
Resource Report
Resource Website 		RRID:Addgene_52347 leu* Saccharomyces cerevisiae Ampicillin PMID:24487693 Backbone Size:5839; Vector Backbone:pST39; Vector Types:Bacterial Expression, p1 Integration Vector; Bacterial Resistance:Ampicillin changed S2F, A3V, Q180* 2026-08-15 01:16:22 0

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