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Species: Homo sapiens
Genetic Insert: caspase-2 prodomain
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBIFC-VN173; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19782032
Proper citation: RRID:Addgene_49262 Copy
Species: Bos taurus
Genetic Insert: Akt
Vector Backbone Description: Vector Backbone:MSCV 2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11135576
Comments: Please note that Addgene's sequencing results identified N2D, D149E and A478G mutations in the Akt sequence in this plasmid when compared to GenBank reference sequence AAW71957.1. These mutations were not intentional and the plasmid functions as described in the associated publication (i.e. it is constitutively active).
Proper citation: RRID:Addgene_49267 Copy
Species: Homo sapiens
Genetic Insert: Eya2
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV 2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49265 Copy
Species: Homo sapiens
Genetic Insert: Caspase-2 CARD
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBiFC-NC155; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19782032
Proper citation: RRID:Addgene_49259 Copy
Species: Homo sapiens
Genetic Insert: Med 26
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21729782
Proper citation: RRID:Addgene_49252 Copy
Species: Homo sapiens
Genetic Insert: Med 26
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21729782
Proper citation: RRID:Addgene_49250 Copy
Species: Homo sapiens
Genetic Insert: Med 26
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21729782
Proper citation: RRID:Addgene_49255 Copy
Species: Homo sapiens
Genetic Insert: Narg2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21729782
Comments: An A75V mutation was found in the insert sequence, it should not effect plasmid function.
Proper citation: RRID:Addgene_49247 Copy
Species: Homo sapiens
Genetic Insert: Med12
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:8753; Vector Backbone:pAC-GHLT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19047373
Proper citation: RRID:Addgene_49240 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49246 Copy
Species: Homo sapiens
Genetic Insert: TET1 tet methylcytosine dioxygenase 1
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALEN, TALE-TET1 Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24108092
Comments: Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596
Proper citation: RRID:Addgene_49239 Copy
Species: Homo sapiens
Genetic Insert: TET1 tet methylcytosine dioxygenase 1
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALEN, TALE-TET1 Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24108092
Comments: Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596
Proper citation: RRID:Addgene_49238 Copy
Species: Homo sapiens
Genetic Insert: TET1 tet methylcytosine dioxygenase 1
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALEN, TALE-TET1 Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24108092
Comments: Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596
Proper citation: RRID:Addgene_49237 Copy
Species: Homo sapiens
Genetic Insert: TALE-TET1CD Backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALEN, TALE-TET1 Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24108092
Comments: Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596
Proper citation: RRID:Addgene_49236 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:8754; Vector Backbone:Plasmid 12255: pWPT-GFP; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49235 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:8754; Vector Backbone:Plasmid 12255: pWPT-GFP; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49234 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:8754; Vector Backbone:Plasmid 12255: pWPT-GFP; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49232 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSFI; Vector Types:Adaptor Plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9843996
Proper citation: RRID:Addgene_49190 Copy
Species: Mus musculus
Genetic Insert: C/EBPbeta (LIP fragment)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pPCR-Script Amp SK(+); Vector Types:Bacterial Expression, cloning vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49194 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSFI; Vector Types:Adaptor Plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9843996
Proper citation: RRID:Addgene_49191 Copy
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