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Species: Danio rerio
Genetic Insert: ZebrafishCommunity-EMP2-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCCCACACAGCACAAGAT
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42710 Copy
Genetic Insert: shLacZ
Vector Backbone Description: Backbone Marker:TRC; Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Proper citation: RRID:Addgene_42559 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-EMP2-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCAGGCAGTGCAGGCCAC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42709 Copy
Species: Homo sapiens
Genetic Insert: EGFR ECD (1-644)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42666 Copy
Species: Homo sapiens
Genetic Insert: EGFR ICD (645-1186)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42667 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16224021
Comments: Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.
Proper citation: RRID:Addgene_42664 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42665 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-oxtr-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGGTGGCGTTTCTGGATG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42794 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-oxtrl-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGATCCTCTGGCCCATTA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42795 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39526 Copy
Species: Homo sapiens
Genetic Insert: ARAP3(R307,8A)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11804589
Proper citation: RRID:Addgene_39486 Copy
Species: Homo sapiens
Genetic Insert: ARAP3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11804589
Proper citation: RRID:Addgene_39484 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21900401
Comments: Please note that the p53 insert in this plasmid contains a known P72R variant when compared to GenBank ID NP_000537.3
Proper citation: RRID:Addgene_39479 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 E38
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Eukaryotic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39514 Copy
Species: Homo sapiens
Genetic Insert: ARID1A
Vector Backbone Description: Vector Backbone:pLenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21900401
Proper citation: RRID:Addgene_39478 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21900401
Comments: Please note that the p53 insert in this plasmid contains a known P72R variant when compared to GenBank ID NP_000537.3
Proper citation: RRID:Addgene_39480 Copy
Species: Homo sapiens
Genetic Insert: HRAS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39503 Copy
Species: Homo sapiens
Genetic Insert: ADP ribosylation factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18524849
Proper citation: RRID:Addgene_39554 Copy
Species: Homo sapiens
Genetic Insert: SRP14
Vector Backbone Description: Vector Backbone:pEGFP-C1P; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18455985
Comments: Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 1540, 1645, 1750 and 1774-80 when compared to the full plasmid sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.
Proper citation: RRID:Addgene_39541 Copy
Species: Mus musculus
Genetic Insert: Akt
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10226029
Proper citation: RRID:Addgene_39531 Copy
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