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Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:#110344; Backbone Size:7423; Vector Backbone:pQC MCS IRES G418; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: KGA.wt obtained with V5-6xHis from pcDNA3.1D V5-His-TOPO-KGA.wt (Addgene #110332)
pQC-based γ-Retroviral transfer vector for KGA.wt-V5 expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110390 Copy
Species: Homo sapiens
Genetic Insert: Yes
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110497 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 (HuR)
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110411 Copy
Species: Homo sapiens
Genetic Insert: SOX4
Vector Backbone Description: Backbone Marker:William Sellers; Vector Backbone:pcDNA3-Flag-FKHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16618720
Proper citation: RRID:Addgene_110360 Copy
Vector Backbone Description: Backbone Marker:#42230; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: pX330 with puromycin resistance
For plasmid usage, please see the associated publication (Cong et al. Science. 2013, PMID: 23287718), as well as Ran et al. Nat Protoc. 2013, PMID: 24157548.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_110403 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6278; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110401 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:#21916; Backbone Size:10959; Vector Backbone:Tet-pLKO-neo; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31040181
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110470 Copy
Species: Homo sapiens
Genetic Insert: Rab5C-S35N
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110504 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:7583; Vector Backbone:pCMV histidinol; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: pCMV human IRS-1 was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into pCMV human IRS-1. Select cells expressing plasmid with approximately 5mM histidinol (Sigma), depending on the cell type.
Proper citation: RRID:Addgene_11238 Copy
Species: Homo sapiens
Genetic Insert: TFE3 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: K562. Use with plasmid pTFE3.1.0-gDNA.
Proper citation: RRID:Addgene_112377 Copy
Species: Homo sapiens
Genetic Insert: TFE3
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTFE3-donor.
Proper citation: RRID:Addgene_112473 Copy
Species: Adenovirus
Genetic Insert: AdML-M3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:pSP72; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215496
Comments: Reed lab clone #388.
Proper citation: RRID:Addgene_11244 Copy
Species: Homo sapiens
Genetic Insert: CUX1
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pCUX1-donor.
Proper citation: RRID:Addgene_112434 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112592 Copy
Species: Synthetic
Genetic Insert: ProD gfpmut3 TT
Vector Backbone Description: Vector Backbone:pFPV25.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29201859
Proper citation: RRID:Addgene_112518 Copy
Vector Backbone Description: Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112580 Copy
Species: Homo sapiens
Genetic Insert: cathepsin B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16339146
Comments: See author's map for more information.
Proper citation: RRID:Addgene_11249 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112588 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112589 Copy
Vector Backbone Description: Backbone Size:5270; Vector Backbone:pRS410; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: pRS400 + pRS415 --> pRS410. Insert is KanMX (G418 resistance) from AatII-cut pRS400. Backbone is ApaLI-cut pRS415 (CEN plasmid).
Proper citation: RRID:Addgene_11258 Copy
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