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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCMX-Gal4-TEAD3
 
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RRID:Addgene_33106 TEAD3 Homo sapiens Ampicillin PMID:18579750 Backbone Size:4500; Vector Backbone:PCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:52 2
pCMV-Flag-YAP-R58A
 
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RRID:Addgene_33061 YAP Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R58A 2026-08-15 01:13:52 0
pCMV-Flag-YAP-R89A
 
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RRID:Addgene_33058 YAP Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R89A 2026-08-15 01:13:52 0
pCMV-Flag-YAP-L68A
 
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RRID:Addgene_33059 YAP Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin L68A 2026-08-15 01:13:46 0
pGEX-KG-GST-YAP-S127A
 
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RRID:Addgene_33053 YAP Homo sapiens Ampicillin PMID:20123905 Backbone Size:5000; Vector Backbone:PGEX-KG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S127A 2026-08-15 01:13:46 0
pRK5-Myc-TEAD1-Y406A
 
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RRID:Addgene_33047 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Y406A 2026-08-15 01:13:46 1
pRK5-Myc-TEAD1-V242A
 
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RRID:Addgene_33048 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin V242A 2026-08-15 01:13:52 0
pRK5-Myc-TEAD1-E205K
 
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RRID:Addgene_33042 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E205K 2026-08-15 01:13:51 0
pRK5-Myc-TEAD1-D278K
 
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RRID:Addgene_33043 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D278K 2026-08-15 01:13:46 0
pCMX-Gal4-TEAD1-I247A
 
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RRID:Addgene_33036 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin I247A 2026-08-15 01:13:46 0
pCMX-Gal4-TEAD1-Y406A
 
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RRID:Addgene_33034 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Y406A 2026-08-15 01:13:51 0
pCMX-Gal4-TEAD1-L272A
 
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RRID:Addgene_33037 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin L272A 2026-08-15 01:13:51 0
pCMX-Gal4-TEAD1-V391A
 
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RRID:Addgene_33038 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin V391A 2026-08-15 01:13:46 0
pCS2+MT-hToca-1-W518K
 
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1+ mentions
RRID:Addgene_33031 Toca-1 Homo sapiens Ampicillin PMID:15260990 Molecular cloning of Toca-1 was difficult, because the cDNA encoding human Toca-1 is lethal to E. coli. The toxicity problem was not resolved by using vectors with low copy numbers or with minimal transcriptional activity, suggesting that the source of toxicity was not the translated protein product but rather the DNA sequence itself. The toxic region of the Toca-1 cDNA was localized to codon 127. The Xenopus tropicalis homolog of Toca-1 is completely nontoxic when cloned using E. coli and is identical in amino acid sequence to human Toca-1 between amino acids 124 and 127, but these amino acids are encoded by different codons. Replacing codons 126 and 127 in the human Toca-1 with the X. Tropicalis sequence (which constitute silent mutations) completely ameliorated the toxicity problem and allowed us to propagate the human Toca-1 clone in E. coli without difficulty. Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin W518K 2026-08-15 01:13:51 1
pCS2+MT-hToca-1-wt
 
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1+ mentions
RRID:Addgene_33030 Toca-1 Homo sapiens Ampicillin PMID:15260990 Molecular cloning of Toca-1 was difficult, because the cDNA encoding human Toca-1 is lethal to E. coli. The toxicity problem was not resolved by using vectors with low copy numbers or with minimal transcriptional activity, suggesting that the source of toxicity was not the translated protein product but rather the DNA sequence itself. The toxic region of the Toca-1 cDNA was localized to codon 127. The Xenopus tropicalis homolog of Toca-1 is completely nontoxic when cloned using E. coli and is identical in amino acid sequence to human Toca-1 between amino acids 124 and 127, but these amino acids are encoded by different codons. Replacing codons 126 and 127 in the human Toca-1 with the X. Tropicalis sequence (which constitute silent mutations) completely ameliorated the toxicity problem and allowed us to propagate the human Toca-1 clone in E. coli without difficulty. Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:46 5
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
 
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RRID:Addgene_33255 PIK3CA Homo sapiens Ampicillin PMID:21876152 Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein" Backbone Size:5168; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin changed histidine 1047 to arginine and phenylalanine 486 to serine; deleted amino acids 343-346 2026-08-15 01:13:54 0
pBabe puro-HA-PIK3CA(H1047R;G320E)
 
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RRID:Addgene_33254 PIK3CA Homo sapiens Ampicillin PMID:21876152 Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein" Backbone Size:5168; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin changed histidine 1047 to arginine and glycine 320 to glutamic acid 2026-08-15 01:13:48 0
HT-ELK1
 
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RRID:Addgene_33258 ELK1 Homo sapiens Kanamycin Backbone Marker:Promega; Backbone Size:5051; Vector Backbone:pFC14K; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:48 0
pBabe puro-eIF4E
 
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RRID:Addgene_33252 eIF4E Homo sapiens Ampicillin PMID:21876152 Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin unmodified 2026-08-15 01:13:54 2
pcDNA1 Flag ATXN1[85Q]-S776A
 
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RRID:Addgene_33240 Ataxin-1 Homo sapiens Ampicillin PMID:17190598 Backbone Marker:Invitrogen; Vector Backbone:pcDNA1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin insert contains a stretch of 85 Glutamines and no stop codon and a S776A mutation 2026-08-15 01:13:48 0

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