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Species: Saccharomyces cerevisiae
Genetic Insert: FPPS
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36990382
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36990382
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36990382
Comments: deadFPPS sequence also contains G253D mutation that was inadvertently introduced during cloning.
Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pIME2
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191793 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pSPR3
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191788 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pSPO11
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191767 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(G)
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182477 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS(M)
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS(M)-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ago- Dcr
Vector Backbone Description: Backbone Size:7959; Vector Backbone:pESC; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: AGO1 and DCR1 genes were amplified from S. castellii gDNA using primersatac gcggccgc aacgatgtcatccaattcgg+ and atac gcggccgc agcacataaactctttgttc, respectively.
Plasmid pESC-LEU was converted to leu2::NAT derivative via short-homology in vivo replacement using PCR product generated using primers AAGCAAGGATTTTCTTAACTTCTTCGGCGACAGCATCACCGACTTCGGTGc agc tga agc ttc gta cgc+tttttccaataggtggttagcaatcgtcttactttctaacttttcttacct agg cca cta gtg gat ctg and pAG25 template.
AGO1 was cloned into NotI site of pESC-NAT to yield pESC-NAT-AGO1 plasmid.
DCR1 was cloned into ApaI/XhoI site of pESC-NAT-AGO1 to yield pESC-NAT-AGO1-DCR1 plasmid.
Proper citation: RRID:Addgene_160381 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: N1199
Vector Backbone Description: Backbone Size:4984; Vector Backbone:PSV3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The plasmid was constructed by gene synthesis and cloning by Genewiz.
Proper citation: RRID:Addgene_160386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PDE1
Vector Backbone Description: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:3825; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note plasmid contains the following mutations T114A, K153E, I201T and N306S in PDE1. Depositor confirms plasmid is functional.
Proper citation: RRID:Addgene_160388 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: L-A Gag-Pol
Vector Backbone Description: Backbone Marker:J Virol . 1993 May;67(5):2764-71. doi: 10.1128/JVI.67.5.2764-2771.1993.; Backbone Size:9854; Vector Backbone:PJD1223; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: pJD1223-NAT was constructed by short-homology in vivo replacement of TRP1 on pJD1223 (J Virol
. 1993 May;67(5):2764-71) with NAT PCR product generated using primers TATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTcagctgaagcttcgtacgc and
caagtgcacaaacaatacttaaataaatactactcagtaataacctattttaggccactagtggatctg from pAG25.
Proper citation: RRID:Addgene_160390 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195039 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2417; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 sequence was amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 14x PP7 with loxP-kanMX-loxP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31101674
Proper citation: RRID:Addgene_189939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-GFPEnvy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37824008
Proper citation: RRID:Addgene_189940 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAK10
Vector Backbone Description: Backbone Size:8743; Vector Backbone:pCAS-mak10 gRNA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: MAK10 was deleted in an S288C background with Cre-loxP. Lox-ed mak10 "deletion " fragment" consisting of sequences upstream and downstream of deleted ORF (~800bp) was amplified, adding XhoI restriction sites, and cloned into the vector. This insert serves as a donor template for making a MAK10 deletion. The vector is an all-in-one gRNA/Cas9/donor template vector for making a marker-free MAK10 deletion in yeast strains
Proper citation: RRID:Addgene_160384 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PCP-NLS-GFPEnvy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37127821
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.15.472793v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_189941 Copy
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