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Species: Homo sapiens
Genetic Insert: PARP1delWGR-L713F
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-520...661-1014 (protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol)
Proper citation: RRID:Addgene_173941 Copy
Species: Homo sapiens
Genetic Insert: PARP1-CAT
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 661-1014
Proper citation: RRID:Addgene_173943 Copy
Species: Homo sapiens
Genetic Insert: PARP1-E988K
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014
Proper citation: RRID:Addgene_173945 Copy
Species: Homo sapiens
Genetic Insert: p300 peak 1 from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173947 Copy
Species: Homo sapiens
Genetic Insert: PARP1delZn2delBRCT
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-96...214-373...521-1014
Proper citation: RRID:Addgene_173939 Copy
Species: Homo sapiens
Genetic Insert: PARP1delZn3
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-213...374-1014
Proper citation: RRID:Addgene_173936 Copy
Species: Homo sapiens
Genetic Insert: Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173965 Copy
Species: Homo sapiens
Genetic Insert: Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Comments: Please note: The plasmid contains a T to C nucleotide mutation in the Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45 compared to the depositor's provided sequence.
Proper citation: RRID:Addgene_173966 Copy
Species: Homo sapiens
Genetic Insert: Human minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173967 Copy
Species: Homo sapiens
Genetic Insert: Human minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173969 Copy
Species: Homo sapiens
Genetic Insert: Enhancer region from COL2A1 gene
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173961 Copy
Species: Homo sapiens
Genetic Insert: Enhancer region 5 (S5) from SOX9 enhancer cluster EC1.25
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173959 Copy
Species: Homo sapiens
Genetic Insert: Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173952 Copy
Species: Homo sapiens
Genetic Insert: Enhancer regions 1 and 2 (S1 and S2) from SOX9 enhancer cluster EC1.35
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173953 Copy
Species: Homo sapiens
Genetic Insert: Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173956 Copy
Species: Homo sapiens
Genetic Insert: Enhancer region 4 (S4) from SOX9 enhancer cluster EC1.25
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173958 Copy
Species: Homo sapiens
Genetic Insert: Minimal enhancer region 1 (min1) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173950 Copy
Species: Homo sapiens
Genetic Insert: AMBRA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174153 Copy
Species: Homo sapiens
Genetic Insert: CCND1
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:VectorBuilder pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174155 Copy
Species: Homo sapiens
Genetic Insert: AMBRA1
Vector Backbone Description: Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174157 Copy
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