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Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pCru5; Vector Types:Mammalian Expression, Retroviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49226 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pCru5; Vector Types:Mammalian Expression, Retroviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49220 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSFI; Vector Types:Adaptor Plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9843996
Proper citation: RRID:Addgene_49186 Copy
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24215641
Comments:
Note that there are two adenines in Addgene's T7 quality control sequence that introduce a HindIII site and an AflII site into the plasmid. Note that these discrepancies with the depositor sequence do NOT affect the function of the plasmid. The vector is used as a template for PCR to amplify DNA cassettes to modify chromosomal gene loci. The additional adenines are not within the primer sequences to amplify DNA cassettes for gene manipulation.
Proper citation: RRID:Addgene_49184 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pCru5; Vector Types:Mammalian Expression, Retroviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49222 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSFI; Vector Types:Adaptor Plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9843996
Proper citation: RRID:Addgene_49188 Copy
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24215641
Proper citation: RRID:Addgene_49182 Copy
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24215641
Proper citation: RRID:Addgene_49181 Copy
Species: Homo sapiens
Genetic Insert: Tim3
Vector Backbone Description: Backbone Size:6094; Vector Backbone:pCDEF3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807895
Proper citation: RRID:Addgene_49212 Copy
Species: Mus musculus
Genetic Insert: Tim3 T1 truncation
Vector Backbone Description: Vector Backbone:pCDEF3-CD8SS-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807895
Proper citation: RRID:Addgene_49210 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49291 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49290 Copy
Genetic Insert: dsRED
Vector Backbone Description: Backbone Size:3422; Vector Backbone:psl1180; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Addgene's quality control sequencing finds a one nucleotide discrepancy in the Aedes aegypti PUb promoter. This discrepancy does not affect promoter function, and the plasmid should work as described.
Proper citation: RRID:Addgene_49327 Copy
Species: Homo sapiens
Genetic Insert: Erk1
Vector Backbone Description: Vector Backbone:p3xFLAG-CMV7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49329 Copy
Species: Homo sapiens
Genetic Insert: Erk1
Vector Backbone Description: Vector Backbone:p3xFLAG-CMV7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49328 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49289 Copy
Species: Synthetic
Genetic Insert: FRT forward
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49310 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49303 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49302 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49309 Copy
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