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Species: Mus musculus
Genetic Insert: Akt
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10226029
Comments: mAkt-HA-ER was cloned from pWZLneo-mAkt-HA-ER from kohn et al(1998) JBC 273(19)11937-11943.
Proper citation: RRID:Addgene_39530 Copy
Species: Drosophila melanogaster
Genetic Insert: CG11798
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_39679 Copy
Species: Danio rerio
Genetic Insert: zebrafish gfap promoter- EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4200; Vector Backbone:pEGFP-1; Vector Types:zebrafish expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16765104
Proper citation: RRID:Addgene_39761 Copy
Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_39818 Copy
Species: Homo sapiens
Genetic Insert: FZR1
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX-6T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note that this construct has an additional mutation in Cdh1 - R52G.
Proper citation: RRID:Addgene_39877 Copy
Species: Homo sapiens
Genetic Insert: CyclinB1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11907280
Proper citation: RRID:Addgene_39871 Copy
Species: Homo sapiens
Genetic Insert: CyclinB1(R42A)
Vector Backbone Description: Vector Backbone:pVenus-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070128
Proper citation: RRID:Addgene_39873 Copy
Genetic Insert: iRFP protein GAF domain (mutated)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23891149
Proper citation: RRID:Addgene_39868 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pYD1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39865 Copy
Genetic Insert: iRFP protein PAS domain
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23891149
Proper citation: RRID:Addgene_39867 Copy
Genetic Insert: iRFP protein PAS domain
Vector Backbone Description: Backbone Marker:Wegerer, A. et al., BMC Biotechnol 8, 2 (2008); Vector Backbone:pWA21 (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23891149
Proper citation: RRID:Addgene_39866 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin
Vector Backbone Description: Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Comments: Please note the YPet fluorescent protein present in the vector backbone is not expressed with the streptavidin insert.
Proper citation: RRID:Addgene_39860 Copy
Species: Mus musculus
Genetic Insert: Neurotrophin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39981 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin, EGFP, TM helix
Vector Backbone Description: Backbone Marker:Life Technology; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39863 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin fused to EGFP
Vector Backbone Description: Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39862 Copy
Species: Rattus norvegicus
Genetic Insert: TrkB.T1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI.
See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., https://www.ncbi.nlm.nih.gov/pubmed?term=1846020 ) to learn more about the TrkB.T1 variant.
Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32
Proper citation: RRID:Addgene_39980 Copy
Species: Homo sapiens
Genetic Insert: hSlp2-a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40056 Copy
Species: Homo sapiens
Genetic Insert: hSlp2-a C2AB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40051 Copy
Species: Homo sapiens
Genetic Insert: Emi1
Vector Backbone Description: Vector Backbone:pT7-Venus-C1; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17485488
Proper citation: RRID:Addgene_39854 Copy
Species: Rattus norvegicus
Genetic Insert: TrkA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide
Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30
Proper citation: RRID:Addgene_39977 Copy
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