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  • Organism:mus musculus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pLenti-NIrD-Mefv iso1
 
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RRID:Addgene_92108 Mefv isoform1 Mus musculus Ampicillin PMID:27482109 Vector Backbone:pLenti-NIrD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:22:27 0
pGAD-GH-Mefv iso1
 
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RRID:Addgene_92107 Mefv isoform1 Mus musculus Ampicillin PMID:27482109 Vector Backbone:pGAD-GH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:27 0
pCS2-3HA-Mefv iso1
 
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RRID:Addgene_92105 Mefv isoform1 Mus musculus Ampicillin PMID:27482109 Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:27 0
pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
 
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1+ mentions
RRID:Addgene_92140 AID[71-114]-eGFP Mus musculus Ampicillin PMID:28525758 use with pX335-EN475 and pX335-EN477 (Cas9nickase + paired sgRNA against mouse CTCF stop codon) Vector Backbone:pEN84 (Addgene #86230); Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:22:28 3
PB-Haunt E1-PuroT2AmCherry-4A KI
 
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RRID:Addgene_92164 Haunt P-Inr2-cDNA KI homology arms Mus musculus Ampicillin PMID:25891907 HA inserts are 472 and 572. Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Vector Backbone:PiggyBac; Vector Types:Other; Bacterial Resistance:Ampicillin 2026-08-15 01:22:28 0
CMV-sgE1TSS-short-3'box
 
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RRID:Addgene_92165 Evx1as short isoform Mus musculus Ampicillin PMID:26996597 Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Vector Backbone:pcDNA3.1; Vector Types:CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:22:28 0
PB-CAG-HRKI(Haunt P-Inr2-cDNA KI)
 
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RRID:Addgene_92163 Haunt CAG KI homology arms Mus musculus Ampicillin PMID:25891907 HA inserts are 497 and 552bp. Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Vector Backbone:PiggyBac; Vector Types:Other; Bacterial Resistance:Ampicillin 2026-08-15 01:22:28 0
pflareA-psd95-exon18.
 
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RRID:Addgene_90249 psd95-exon18 Mus musculus Kanamycin PMID:23636947 Vector Backbone:pflareA; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:05 0
pL452(hygro)-Sf3b1-K700K
 
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RRID:Addgene_90426 Sf3b1 - left and right homology arms Mus musculus Ampicillin PMID:30462273 Backbone Size:4823; Vector Backbone:pL452; Vector Types:homology-directed repair vector; Bacterial Resistance:Ampicillin 2026-08-15 01:22:07 0
pL452-Sf3b1-K700E
 
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RRID:Addgene_90425 Sf3b1 - left and right homology arms Mus musculus Ampicillin PMID:30462273 Backbone Size:4823; Vector Backbone:pL452; Vector Types:homology-directed repair vector; Bacterial Resistance:Ampicillin 2026-08-15 01:22:07 0
sgSf3b1(T1)
 
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RRID:Addgene_90424 mus Sf3b1 gRNA Mus musculus Ampicillin PMID:30462273 Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:Mammalian Expression, CRISPR, guideRNA encoding for gene editing; Bacterial Resistance:Ampicillin 2026-08-15 01:22:07 0
pBABE-hygro p53 DD
 
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10+ mentions
RRID:Addgene_9058 p53 DD Mus musculus Ampicillin PMID:11884599 Backbone Size:5558; Vector Backbone:pBABE hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin DD. Dominant negative mutant carries deletion of 288 amino acids (D15-301), leaving initial 14aa and the oligomerization and COOH-domains. 2026-08-15 01:22:08 19
pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)
 
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1+ mentions
RRID:Addgene_44027 mHIF-2α (P405A/P530V/N851A) Mus musculus Ampicillin PMID:17804822 This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α. Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites. A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein. This plasmid has an A94V polymorphism that does not affect protein function. Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin P405A, P530V, N851A 2026-08-15 01:15:11 7
pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)
 
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10+ mentions
RRID:Addgene_44028 mHIF-1α (P402A/P577A/N813A) Mus musculus Ampicillin PMID:17804822 This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-1α. Full-length murine HIF-1α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites. A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-1α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 402, Proline 577 and Asparagine 813 to alanines. The newly constructed plasmid was sequenced to confirm encoding of the correct protein. Alternate plasmid names: HIF-1α triple mutant (TM); pcDNA3 HIF-1α TM Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin P402A, P577A, N813A 2026-08-15 01:15:12 16
N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS
 
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1+ mentions
RRID:Addgene_43969 Cbx5 Mus musculus Ampicillin PMID:22704655 Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin N-term chromo domain removed 2026-08-15 01:15:11 1
pCR3-FLAG-TRAF6-C70A
 
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1+ mentions
RRID:Addgene_43926 TNF Receptor-Associated Factor 6 Mus musculus Ampicillin PMID:17135271 Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C70A 2026-08-15 01:15:11 1
pRK6-HA-TAK1
 
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1+ mentions
RRID:Addgene_44160 Tak1 Mus musculus Ampicillin PMID:16260783 Vector Backbone:pRK6-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:13 2
K15/pGL3
 
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1+ mentions
RRID:Addgene_44264 K15 promoter (-4.8) Mus musculus Ampicillin PMID:14708593 Alternate plasmid name: K15(-4.8)-pGL3 The K15 promoter was cloned from C57 BL/SJ mouse genomic DNA (isolated from tail) by PCR using the Supermix High Fidelity Kit (GIBCO). The sequences of the forward and reverse primers were CTGAGCTACCAGCGAGACTCC (K15F1) and TTCCTGTCCCTAGCAAGCAGGAGAG (K15R1), respectively. XhoI and EcoRI restriction sequences were added to the 5' end of forward and reverse primers respectively for subsequent cloning of the PCR product into PBK/CMV vector (Stratagene). The promoter fragment was then subcloned into pGL3-Basic (Promega). The entire K15 promoter-luciferase transgene can be released by digestion with XhoI/SalI. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Mouse Targeting, Luciferase; Bacterial Resistance:Ampicillin Contains the murine K15 promoter (-4.8) fragment 2026-08-15 01:15:13 1
K15-TK/pGL3
 
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RRID:Addgene_44267 K15 promoter (-4.8) Mus musculus Ampicillin PMID:16288281 Alternate plasmid name: K15-HSV-TK-pGL3 The K15 promoter was cloned from C57 BL/SJ mouse genomic DNA (isolated from tail) by PCR using the Supermix High Fidelity Kit (GIBCO). The sequences of the forward and reverse primers were CTGAGCTACCAGCGAGACTCC (K15F1) and TTCCTGTCCCTAGCAAGCAGGAGAG (K15R1), respectively. XhoI and EcoRI restriction sequences were added to the 5' end of forward and reverse primers respectively for subsequent cloning of the PCR product into PBK/CMV vector (Stratagene). The promoter fragment was then subcloned into pGL3-Basic (Promega) to create plasmid K15(-4.8)-pGL3 (Addgene plasmid #44264). The HSV-1 TK reporter gene cDNA was then excised from a TK/PBSIIKS plasmid, using NotI and SalI, and cloned downstream of the K15 promoter, replacing luciferase. The entire K15 promoter-HSV-TK transgene can be released by digestion with XhoI/SalI. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Mouse Targeting, Thymidine kinase; Bacterial Resistance:Ampicillin Contains the murine K15 promoter (-4.8) fragment 2026-08-15 01:15:14 0
K15-EGFP
 
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RRID:Addgene_44266 K15 promoter (-4.8) Mus musculus Kanamycin PMID:15024388 Alternate plasmid name: pEGFP-N2 K15 The K15 promoter was cloned from C57 BL/SJ mouse genomic DNA (isolated from tail) by PCR using the Supermix High Fidelity Kit (GIBCO). The sequences of the forward and reverse primers were CTGAGCTACCAGCGAGACTCC (K15F1) and TTCCTGTCCCTAGCAAGCAGGAGAG (K15R1), respectively. XhoI and EcoRI restriction sequences were added to the 5' end of forward and reverse primers respectively for subsequent cloning of the PCR product into PBK/CMV vector (Stratagene). The promoter fragment was then subcloned into pGEGFP-N2 The entire K15 promoter-EGFP transgene can be released by digestion with XhoI/AflII. Backbone Marker:Clontech; Backbone Size:4737; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression, Mouse Targeting, Fluorescence microscopy; Bacterial Resistance:Kanamycin Contains the murine K15 promoter (-4.8) fragment 2026-08-15 01:15:14 0

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