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Species: Homo sapiens
Genetic Insert: PKM1
Vector Backbone Description: Backbone Marker:ATCC#87362 (PMID:7737504); Backbone Size:5557; Vector Backbone:P413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21789790
Proper citation: RRID:Addgene_34607 Copy
Species: Homo sapiens
Genetic Insert: SENP3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19015314
Proper citation: RRID:Addgene_34554 Copy
Species: Homo sapiens
Genetic Insert: B23/nucleophosmin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4600; Vector Backbone:pCMV-DsRed-Express; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19015314
Comments: Addgene quality control sequencing found an A123V point mutation, which, according to the depositing lab, should not alter the plasmid's function.
Proper citation: RRID:Addgene_34553 Copy
Species: Homo sapiens
Genetic Insert: L57
Vector Backbone Description: Backbone Marker:PMID: 12864922 ; Backbone Size:5000; Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: tandem fusion of LOV2 domain from A. Sativa phototropin 1, the catalytic domain of caspase-7 (human), and a mRFP variant that contains T140A and M141S mutations (introduced to facilitate cloning).
Proper citation: RRID:Addgene_34557 Copy
Species: Homo sapiens
Genetic Insert: SENP5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19015314
Proper citation: RRID:Addgene_34555 Copy
Species: Homo sapiens
Genetic Insert: pLyn-CaRQ
Vector Backbone Description: Backbone Marker:PMID: 12864922 ; Backbone Size:5000; Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22195563
Comments: LYN membrane anchor - MGCIKSKGKDSATS
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL
Proper citation: RRID:Addgene_34559 Copy
Species: Homo sapiens
Genetic Insert: Wnt10B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34748 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34695 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34694 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34693 Copy
Species: Homo sapiens
Genetic Insert: TNKS2-M1054V
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11802774
Proper citation: RRID:Addgene_34692 Copy
Species: Homo sapiens
Genetic Insert: Sorting nexin 9
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17988218
Proper citation: RRID:Addgene_34690 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34683 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1
Vector Backbone Description: Vector Backbone:pUHD10-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7559787
Proper citation: RRID:Addgene_34688 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34682 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15004222
Proper citation: RRID:Addgene_34681 Copy
Species: Homo sapiens
Genetic Insert: PTD012
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34797 Copy
Species: Homo sapiens
Genetic Insert: CD68 promoter/enhancer -680/+140
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11907098
Comments: The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68.
Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_34837 Copy
Species: Homo sapiens
Genetic Insert: HORMA domain containing protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34821 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037171
Proper citation: RRID:Addgene_34705 Copy
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