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Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9804821
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag and a K403A mutation in the PI-SceI ORF.
Proper citation: RRID:Addgene_195612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Small ubiquitin-like modifier
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6706; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37020212
Comments: Please hold for publication.
Proper citation: RRID:Addgene_190676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDS1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184760 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAH1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184761 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DGK1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184759 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Partial Bem1 C-terminus
Vector Backbone Description: Vector Backbone:pG23A; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37004720
Proper citation: RRID:Addgene_173925 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: N1199
Vector Backbone Description: Backbone Size:3704; Vector Backbone:pAG25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: 2.8 kb dsRNA species gel purified from yJM 1199 and ligated to PC3 oligo (/5Phos/GG ATC CCG GGA ATT CGG TAA TAC GAC TCA CTA TAT TTT TAT AGT GAG TCG TAT TA). After reverse transcription and strand annealing, cDNA amplified with PC2 primer (CCG AAT TCC CGG GAT CC )to yield a 2.8 kb fragment. Fragment phosphorylated and ligated to pAG25 digested with EcoRV and phosphatase treated. Clones checked with restriction digest and sequencing. Near full length N1199 cDNA
Proper citation: RRID:Addgene_160379 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YGL055W
Vector Backbone Description: Backbone Size:6211; Vector Backbone:Yc33 (CEN); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33658354
Proper citation: RRID:Addgene_160653 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: T2A-Flp1
Vector Backbone Description: Vector Backbone:pACGCB(2.1)-Gal4; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36617215
Proper citation: RRID:Addgene_194770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_1-17
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148717 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_1-49
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148718 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_1-49
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148719 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_101-150
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_107-122
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148712 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4G_408-485
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4G, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P39935) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148713 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4G_448-500
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4G, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P39935) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4E_25-213-KKKKtoAAAA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4E, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P07260) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4E_25-213-I77AI81A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4E, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P07260) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148727 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_123-150
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148814 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pab1
Vector Backbone Description: Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25671604
Comments: pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment. Expresses Pab1 fragment fused to GAL4BD.
Proper citation: RRID:Addgene_196834 Copy
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