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Species: Synthetic
Genetic Insert: ZhcD-Cit-tevs-DHFR
Vector Backbone Description: Vector Backbone:PB-PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357
Proper citation: RRID:Addgene_116051 Copy
Species: Synthetic
Genetic Insert: Casp-rastvTEV-teTVMV2
Vector Backbone Description: Vector Backbone:PB-CMV-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357
Proper citation: RRID:Addgene_116098 Copy
Species: Synthetic
Genetic Insert: HSVPZ
Vector Backbone Description: Vector Backbone:CMV-TO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357
Proper citation: RRID:Addgene_116101 Copy
Species: Synthetic
Genetic Insert: SMVPZ
Vector Backbone Description: Vector Backbone:CMV-TO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357
Proper citation: RRID:Addgene_116100 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127729 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127728 Copy
Species: Synthetic
Genetic Insert: Synthetic CRISPR array containing four identical T. thermophilus spacers
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3920; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31278272
Proper citation: RRID:Addgene_127764 Copy
Species: Synthetic
Genetic Insert: Convergent LacI-TetR Toggle
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682205 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127761 Copy
Species: Synthetic
Genetic Insert: AsCpf1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31316073
Proper citation: RRID:Addgene_127750 Copy
Species: Synthetic
Genetic Insert: mSpinach,RFP Divergent Orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682222 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127756 Copy
Species: Synthetic
Genetic Insert: mSpinach, MG RNA aptamer in divergent orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682223 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127759 Copy
Species: Synthetic
Genetic Insert: mSpinach, MG RNA aptamer in convergent orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682223 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127758 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127746 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127745 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127744 Copy
Species: Synthetic
Genetic Insert: nourseothricin N-acetyltransferase
Vector Backbone Description: Vector Backbone:pDSM-ab; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: NatMX transcription unit with pathway position 2 homology arms, compatible with 5' position 1 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127747 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B4 VP1 gene
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32313222
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_127849 Copy
Species: Synthetic
Genetic Insert: 4 repeats of COX8 signal peptide plus GCaMP6f
Vector Backbone Description: Backbone Size:5433; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31862210
Proper citation: RRID:Addgene_127870 Copy
Species: Synthetic
Genetic Insert: Red-shifted mitochondrial matrix Ca2+ indicator
Vector Backbone Description: Backbone Size:5452; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31862210
Proper citation: RRID:Addgene_127873 Copy
Species: Synthetic
Genetic Insert: OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
Vector Backbone Description: Backbone Size:5394; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31862210
Proper citation: RRID:Addgene_127871 Copy
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