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Species: Saccharomyces cerevisiae
Genetic Insert: TPS1, GSY2
Vector Backbone Description: Vector Backbone:pROS13; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Cloning details are in the Methods section and Supplementary Table 2 of the paper.
Proper citation: RRID:Addgene_196612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CAN1 gRNA
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_182521 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4FLAG N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193315 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468518
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag.
Proper citation: RRID:Addgene_195610 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18644379
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K122I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus.
Proper citation: RRID:Addgene_195614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4FLAG N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193317 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4MYC N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193318 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18644379
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The protein contains a carboxyl-terminal histidine tag for purification. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur).
Proper citation: RRID:Addgene_195613 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EMC1
Vector Backbone Description: Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184763 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABF2
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184768 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: IRE1
Vector Backbone Description: Vector Backbone:UG6-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184766 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CIT1
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184767 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AFG3
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OM14
Vector Backbone Description: Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184758 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CWP2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212919 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212918 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUC2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212915 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SCW4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212913 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP150
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212912 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212929 Copy
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