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Species: Synthetic
Genetic Insert: 3xFLAG
Vector Backbone Description: Backbone Size:4578; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127862 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.N VP1 gene
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32313222
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_127851 Copy
Species: Synthetic
Genetic Insert: PDZ-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127961 Copy
Species: Synthetic
Genetic Insert: AAVS1-CAG-KRAB-dCas9-BFP-KRAB
Vector Backbone Description: Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127966 Copy
Species: Synthetic
Genetic Insert: Myr-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127963 Copy
Species: Synthetic
Genetic Insert: CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
Vector Backbone Description: Vector Backbone:pC13N; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127969 Copy
Species: Synthetic
Genetic Insert: AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
Vector Backbone Description: Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127967 Copy
Species: Synthetic
Genetic Insert: sYFP2-OP18-mScI-R125I
Vector Backbone Description: Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128002 Copy
Species: Synthetic
Genetic Insert: Myr-3xSAG-Dr-hel4-TrkB
Vector Backbone Description: Vector Backbone:pCMVd2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30850602
Comments: Please note that there are some discrepancies between Addgene's NGS sequence and the depositor's provided partial sequence. The depositor noted that these discrepancies do not affect plasmid function.
Proper citation: RRID:Addgene_127955 Copy
Species: Synthetic
Genetic Insert: ROPY-sYFP2(Y67G)
Vector Backbone Description: Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128008 Copy
Species: Synthetic
Genetic Insert: iGlucoSnFR-TS
Vector Backbone Description: Vector Backbone:pAAV.CAG...WPRE.SV40pA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31106909
Comments: Commercial use is protected by US Patent 9939437
Proper citation: RRID:Addgene_127952 Copy
Species: Synthetic
Genetic Insert: EB3-iRFP713
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128007 Copy
Species: Synthetic
Genetic Insert: Myr-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pCMVd2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127957 Copy
Species: Synthetic
Genetic Insert: Myr-3xSAG-Dr-hel4-TrkA
Vector Backbone Description: Vector Backbone:pCMVd2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127956 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10152; Vector Backbone:pAC575; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127984 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10868; Vector Backbone:pAC573; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Proper citation: RRID:Addgene_127982 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10239; Vector Backbone:pAC572; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127981 Copy
Species: Synthetic
Genetic Insert: Green fluorescence protein
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2679; Vector Backbone:pMD19 T-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31963386
Proper citation: RRID:Addgene_128141 Copy
Species: Synthetic
Genetic Insert: mplum
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128071 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Vector Backbone:pLVX TRE 3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128070 Copy
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