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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pROS_phleo-Tps1/Gsy2
 
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RRID:Addgene_196612 TPS1, GSY2 Saccharomyces cerevisiae Ampicillin PMID:36849832 Cloning details are in the Methods section and Supplementary Table 2 of the paper. Vector Backbone:pROS13; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:26:02 0
CAN1 gRNA HIS
 
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RRID:Addgene_182521 CAN1 gRNA Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:00 0
HYG-TET-OFF – ubiquitin – LEU – 4FLAG
 
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RRID:Addgene_193315 Ubiquitin - Leucine - 4FLAG N-terminal tagging Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:02 0
pET PI-SceI C-His
 
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RRID:Addgene_195610 PI-SceI Saccharomyces cerevisiae Ampicillin PMID:9468518 Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag. Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T825C, C900G, A901C, A903C, T1131G, T1137C, T1227C, C1230A, A1231C, T1359G, G657A, T658C, G660A 2026-08-15 01:25:02 0
pET15b I-SceI (Ctermhis) K122I
 
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RRID:Addgene_195614 I-SceI Saccharomyces cerevisiae Ampicillin PMID:18644379 Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K122I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus. Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C2458G, A2826T, A2827T 2026-08-15 01:25:03 0
KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG
 
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RRID:Addgene_193317 Ubiquitin - Leucine - 4FLAG N-terminal tagging Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:03 0
KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC
 
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RRID:Addgene_193318 Ubiquitin - Leucine - 4MYC N-terminal tagging Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:03 0
pET15b I-SceI (Ctermhis -MH)
 
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RRID:Addgene_195613 I-SceI Saccharomyces cerevisiae Ampicillin PMID:18644379 Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The protein contains a carboxyl-terminal histidine tag for purification. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin None 2026-08-15 01:25:06 0
EMC1-2EGFP-ter-NAT
 
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RRID:Addgene_184763 EMC1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 0
ABF2-2EGFP-ter-URA3
 
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RRID:Addgene_184768 ABF2 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:05 0
IRE1-2EGFP-ter-kanMX4
 
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RRID:Addgene_184766 IRE1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG6-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:05 0
CIT1-2EGFP-ter-URA3
 
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RRID:Addgene_184767 CIT1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 0
AFG3-2EGFP-ter-URA3
 
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RRID:Addgene_184770 AFG3 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 0
OM14-2EGFP-UG74-ter
 
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RRID:Addgene_184758 OM14 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:05 0
pYSD082
 
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RRID:Addgene_212919 CWP2 Saccharomyces cerevisiae Chloramphenicol PMID:38483353 Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:28:51 0
pYSD081
 
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RRID:Addgene_212918 AGA2 Saccharomyces cerevisiae Chloramphenicol PMID:38483353 Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:28:51 0
pYSD061
 
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RRID:Addgene_212915 SUC2 Saccharomyces cerevisiae Chloramphenicol PMID:38483353 Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:28:51 0
pYSD043
 
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RRID:Addgene_212913 SCW4 Saccharomyces cerevisiae Chloramphenicol PMID:38483353 Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:28:51 0
pYSD042
 
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RRID:Addgene_212912 HSP150 Saccharomyces cerevisiae Chloramphenicol PMID:38483353 Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:28:51 0
pYSD153
 
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RRID:Addgene_212929 mRuby2 Saccharomyces cerevisiae Ampicillin PMID:38483353 Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:51 0

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