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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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SOX2se-CPG-TV Resource Report Resource Website 1+ mentions |
RRID:Addgene_70155 | 5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se | Synthetic | Ampicillin | PMID:26406378 | Backbone Marker:invitrogen; Backbone Size:4583; Vector Backbone:topo; Vector Types:Mouse Targeting, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:58 | 1 | ||
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Dazl-CPG-TV Resource Report Resource Website |
RRID:Addgene_70144 | 5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL | Synthetic | Ampicillin | PMID:26406378 | Backbone Marker:invitrogen; Backbone Size:4258; Vector Backbone:topo; Vector Types:Mouse Targeting, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:03 | 0 | ||
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GAPDH-CPG-TV Resource Report Resource Website 1+ mentions |
RRID:Addgene_70148 | 5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh | Synthetic | Ampicillin | PMID:26406378 | Backbone Marker:invitrogen; Backbone Size:3952; Vector Backbone:topo; Vector Types:Mouse Targeting, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:58 | 4 | ||
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NSarQ27G-VMAn-FKBP Resource Report Resource Website 1+ mentions |
RRID:Addgene_70230 | alpha Sarcin (N-terminal portion) | Synthetic | Ampicillin | PMID:24481070 | Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Gln 27 to Gly | 2026-08-15 01:18:59 | 1 | |
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pcDNA3.1-sfCherry(1-10) Resource Report Resource Website 1+ mentions |
RRID:Addgene_70222 | sfCherry(1-10) | Synthetic | Ampicillin | PMID:26988139 | Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:59 | 1 | ||
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pWKS1583 Resource Report Resource Website |
RRID:Addgene_70188 | amyEopt | Synthetic | Chloramphenicol | PMID:27333161 | Please note: the depositing lab has determined that a particular IS element present in E. coli strains such as DH5alpha and its derivatives has a high propensity to insert into this plasmid. The depositing lab recommends isolating the plasmid DNA and transforming into a strain similar to MC1061. Publication describing the backbone vector pRPF185 as well as the sequencing primers: Clostridium difficile has two parallel and essential Sec secretion systems. Fagan RP, Fairweather NF. J Biol Chem. 2011 Aug 5;286(31):27483-93. doi: 10.1074/jbc.M111.263889. Epub 2011 Jun 9. PMID: 21659510 | Backbone Marker:Fagan and Fairweather 2009; Backbone Size:7216; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:18:59 | 0 | |
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pcDNA3-NGFP-VMAn-FKBP Resource Report Resource Website 1+ mentions |
RRID:Addgene_70225 | eGFP | Synthetic | Ampicillin | PMID:24481070 | Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Glu 125 to Ile, Deleted amino acids 129-239 | 2026-08-15 01:18:59 | 1 | |
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3XFLAG-NSarQ27G-VMAn-FKBP Resource Report Resource Website 1+ mentions |
RRID:Addgene_70229 | alpha Sarcin (N-terminal portion) | Synthetic | Ampicillin | PMID:24481070 | Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Gln 27 to Gly | 2026-08-15 01:18:59 | 1 | |
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lentiCas9-Venus Resource Report Resource Website 1+ mentions |
RRID:Addgene_70267 | Cas9 | Synthetic | Ampicillin | PMID:26375006 | Subcloned Venus in place of Blast in lentiCas9-Blast. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047 PubMed 25075903 Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan G, Zhang F, Orkin SH, Bauer DE. BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 September 16; in press; doi:10.1038/nature15521. | Backbone Size:9080; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:59 | 1 | |
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lentiGuide-Crimson Resource Report Resource Website 1+ mentions |
RRID:Addgene_70683 | S. pyogenes sgRNA cassette | Synthetic | Ampicillin | PMID:26375006 | Cloned in Crimson Reporter in place of PURO reporter into lentiGuide-Puro. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047 PubMed 25075903 Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan G, Zhang F, Orkin SH, Bauer DE. BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 September 16; in press; doi:10.1038/nature15521. | Backbone Size:9482; Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:06 | 8 | |
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pAAV EF1a DIO iChloC 2A dsRed Resource Report Resource Website 1+ mentions |
RRID:Addgene_70762 | Channelrhodopsin-2 | Synthetic | Ampicillin | PMID:26443033 | Backbone Size:5601; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | E83Q,E90R,E101S,D156N,T159C | 2026-08-15 01:19:05 | 4 | |
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pZY102 Resource Report Resource Website |
RRID:Addgene_73933 | bar | Synthetic | Spectinomycin | PMID:15034747 | This vector is suitable for plant transformation and has been used as standard service empty vector control. | Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:19:33 | 0 | |
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pZY101 Resource Report Resource Website |
RRID:Addgene_73932 | bar | Synthetic | Spectinomycin | PMID:15034747 | This vector is suitable hpRNA-mediated gene silencing in tissue (seed) specific manner. Please see the Plant Transformation Core Facility website for more information on using this plasmid: http://plantsci.missouri.edu/muptcf/pzy101.html The vector has following features: 1. It has two replicate origins that allow to replicate in both E. coli and Agrobacterium tumefaciens cells (at higher copy numbers). 2. It also has bom site that allows you to use tri-parental mating to mobilize the vector to Agrobacterium cells. 3. The aadA locus confers Spectinomycin (100 mg/L) or streptomycin (100 mg/L) resistance and thus enable to use either or both of these two antibiotics for the vector selection. 4. The multiple cloning site (MCS) adjacent to the double CaMV35S promoter will allow you to insert your cassette. This CaMV35S promoter is used to drive the bar ORF and, therefore, is not used in front to drive your gene of interest. You need to assemble your own expression cassette including promoter, gene coding sequence, and terminator, and then insert to anywhere between Hind III and EcoR I within the MCS. Special notes: 1. Insert your gene expression cassette in such a way that your promoter is adjacent to the promoter driving the bar gene selectable marker. This is to prevent transcription read-through from your gene expression cassette. 2. In order to use the EcoR I site within the MCS, a minimal ratio of this enzyme versus DNA is recommended to avoid star activity. When HindIII and EcoRI are in combined use, even less of each enzyme is required, and reaction should be conducted in a large volume to avoid excessive enzymatic activity. | Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:19:33 | 0 | |
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pTU2S-a (colE1 origin) Resource Report Resource Website |
RRID:Addgene_74091 | Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin | Synthetic | Chloramphenicol | PMID:27096716 | Backbone Marker:Terrence Lai (this study); Backbone Size:1878; Vector Backbone:colE1 origin and chloramphenicol resistance; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:19:34 | 0 | ||
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pBP-TL9 Resource Report Resource Website |
RRID:Addgene_74098 | TL9 RBS | Synthetic | Chloramphenicol | PMID:27096716 | This plasmid contains the TL9 RBS upstream of GFP. | Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:19:34 | 0 | |
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pTU2S-b (p15A origin) Resource Report Resource Website |
RRID:Addgene_74089 | Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin | Synthetic | Chloramphenicol | PMID:27096716 | Backbone Marker:Terrence Lai (this study); Backbone Size:2024; Vector Backbone:p15A origin and chloramphenicol resistance; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:19:34 | 0 | ||
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pAL-pyk_NT Resource Report Resource Website |
RRID:Addgene_74072 | sgRNA pyk (NT) | Synthetic | Spectinomycin | PMID:26829286 | Vector Backbone:pAL374; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2026-08-15 01:19:34 | 0 | ||
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pAL-pyk_T Resource Report Resource Website |
RRID:Addgene_74070 | sgRNA pyk (T) | Synthetic | Spectinomycin | PMID:26829286 | Vector Backbone:pAL374; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-15 01:19:34 | 0 | ||
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pAL-rfp_T Resource Report Resource Website |
RRID:Addgene_74075 | sgRNA rfp (T) | Synthetic | Spectinomycin | PMID:26829286 | Vector Backbone:pAL374; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-15 01:19:34 | 0 | ||
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pISceI-myl7:MBIC5-mCer3 Resource Report Resource Website |
RRID:Addgene_74117 | MBIC5-mCer3 | Synthetic | Ampicillin | PMID:26808669 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, mRNA generation for microinjection.; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:35 | 0 |
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