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Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two original binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Flanking CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The inner binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11313 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113088 Copy
Genetic Insert: 1 perfect binding site for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12600936
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
GATCGCCGTG TAA T TCTAGA GTCGGGGCG.
We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.
This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.
All UTR inserts were then constructed in the following format:
TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC.
A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG.
The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
Proper citation: RRID:Addgene_11310 Copy
Species: Synthetic
Genetic Insert: GINKO1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Proper citation: RRID:Addgene_113112 Copy
Species: Homo sapiens
Genetic Insert: RapGEF3
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11801596
Proper citation: RRID:Addgene_113110 Copy
Species: Mus musculus
Genetic Insert: Gata3 donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113119 Copy
Vector Backbone Description: Vector Backbone:T444T; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924347
Comments: For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516
Proper citation: RRID:Addgene_113082 Copy
Species: Caenorhabditis elegans
Genetic Insert: K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16222231
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113061 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA for rat Rosa26
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:9271; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Comments: Note: Addgene NGS unable to fully resolve the CAG promoter sequence. Please refer to the depositor's provided sequence for that section of the plasmid.
Proper citation: RRID:Addgene_113161 Copy
Vector Backbone Description: Backbone Marker:IDT; Backbone Size:1900; Vector Backbone:pIDTKan; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113160 Copy
Genetic Insert: 2 binding sites for let-7a miRNA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: 2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid.
Proper citation: RRID:Addgene_11325 Copy
Species: unknown
Genetic Insert: CD28-PafA
Vector Backbone Description: Vector Backbone:pEF6a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104635
Proper citation: RRID:Addgene_113400 Copy
Vector Backbone Description: Backbone Size:17653; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30240613
Comments: Selectable Marker: Tetracycline; 30 μg/mL in E.coli and 100 μg/mL in Pseudomonas aeruginosa
Proper citation: RRID:Addgene_113347 Copy
Species: Homo sapiens
Genetic Insert: SAM sgRNA scaffold with MS2-hairpin loops
Vector Backbone Description: Vector Backbone:pKLV2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30477585
Proper citation: RRID:Addgene_113345 Copy
Vector Backbone Description: Backbone Size:6936; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30240613
Comments: Selectable Marker: Carbenicillin; 50 μg/mL in E.coli and 150 μg/mL in Pseudomonas aeruginosa
Proper citation: RRID:Addgene_113348 Copy
Species: Yarrowia lipolytica
Genetic Insert: beta-isopropyl malate dehydrogenase
Vector Backbone Description: Vector Backbone:pPS3911; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29792930
Comments: Note: Plasmid contains a T base insertion near the C-terminus of the Leu2 marker. This insertion is not known to affect Leu2 function.
Proper citation: RRID:Addgene_113332 Copy
Species: Homo sapiens
Genetic Insert: PRE
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10757795
Comments: Two copies of a consensus Progesterone Response Element (PRE) upstream of the thymidine kinase promoter.
Proper citation: RRID:Addgene_11350 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113448 Copy
Genetic Insert: FnCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113432 Copy
Species: Haemophilus parainfluenzae
Genetic Insert: type II-C anti-CRISPR
Vector Backbone Description: Backbone Size:4094; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30514786
Proper citation: RRID:Addgene_113436 Copy
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