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Species: Homo sapiens
Genetic Insert: GSK3beta S9A
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23085750
Proper citation: RRID:Addgene_49492 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-1:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002478
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_49411 Copy
Species: Opsanus tau
Genetic Insert: Twitch-2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24390440
Comments: There is a discrepancy in the publication, Plasmid 48203: Twitch-2B pRSETB and Plasmid 49531: Twitch-2B pcDNA3 are misidentified.
Proper citation: RRID:Addgene_49531 Copy
Species: Mus musculus
Genetic Insert: BMPR-1B DN
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9303535
Comments: The BMPR-1B insert in this plasmid contains a L2V mutation when compared to GenBank reference sequence NP_031586.1. This mutation is not a concern for the function of the plasmid, as described in the associated publication.
Proper citation: RRID:Addgene_49530 Copy
Genetic Insert: P750
Vector Backbone Description: Backbone Size:2597; Vector Backbone:pDO12A; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49525 Copy
Species: Homo sapiens
Genetic Insert: KFL6-4A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23085750
Proper citation: RRID:Addgene_49489 Copy
Species: Gallus gallus
Genetic Insert: BMPR-1B DN
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9303535
Comments: The BMPR-1B insert in this plasmid contains P2A, M11V and S18G mutations when compared to GenBank reference sequence NP_990463.1. These mutations are not a concern for the function of the plasmid, as described in the associated publication.
Proper citation: RRID:Addgene_49529 Copy
Species: Gallus gallus
Genetic Insert: BMPR-1B CA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9527884
Comments: The BMPR-1B insert in this plasmid contains P2A, M11V and S18G mutations when compared to GenBank reference sequence NP_990463.1. These mutations are not a concern for the function of the plasmid, as described in the associated publication.
Proper citation: RRID:Addgene_49528 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_49480 Copy
Vector Backbone Description: Backbone Marker:Andrew Fire lab; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23955274
Comments: 725 bp of unc54 3'UTR was used for C. elegans expression.
mutations in pthA: delta 1-152, delta 299-875, delta 949-1163
mutations in ZNF-fokI: E484A and R487S
This vector and Addgene plasmid # 49516 are used for insertion of custom repeats which can be assembled by the unit assembly method.
Proper citation: RRID:Addgene_49514 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_49478 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_49477 Copy
Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24185512
Proper citation: RRID:Addgene_49500 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_49465 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flp(H305L)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9818722
Comments: This plasmid expresses a Flp ‘step-arrest’ mutant, Flp H305L, which carries out the first step of recombination, DNA cleavage, but fails to accomplish subsequent strand exchange and religation (Parsons et al.,1988).
Proper citation: RRID:Addgene_49459 Copy
Species: zygosaccharomyces rouxii
Genetic Insert: recR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6000; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10075889
Proper citation: RRID:Addgene_49457 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_49454 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_49452 Copy
Species: Mus musculus
Genetic Insert: Mrpl11-mod
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19372376
Proper citation: RRID:Addgene_49569 Copy
Species: Erwinia amylovora
Genetic Insert: ECF32_1122
Vector Backbone Description: Vector Backbone:pVRa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49689 Copy
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