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Species: Other
Genetic Insert: Cas9m2
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47314 Copy
Species: Other
Genetic Insert: Cas9m2-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47317 Copy
Species: Other
Genetic Insert: Cas9m3-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47318 Copy
Species: Other
Genetic Insert: Cas9m3
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47315 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-snai1b-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCTTGACAAGAAATGAGC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47272 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-spermassociatedantigen7-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TTCTGGGCTCGATCTTGA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47273 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-smyd1a-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCTCTGGTACCCCGCAGA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47270 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-spermassociatedantigen7-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGATCGTGAACGGTTGGA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47274 Copy
Species: Homo sapiens
Genetic Insert: Amphiphysin Type I
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Alanine 65 missing
Proper citation: RRID:Addgene_47390 Copy
Species: Homo sapiens
Genetic Insert: Rab35 S22N
Vector Backbone Description: Vector Backbone:pcDNA3-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23264734
Proper citation: RRID:Addgene_47429 Copy
Species: Mus musculus
Genetic Insert: KIF17-short
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This vector directs expression of neither of the current mouse RefSeq Kif17 variants, isoform 1 or isoform 2. Rather the vector expresses a cDNA which corresponds to a different alternatively spliced transcript encoding a still shorter isoform of 511 amino acids rather than the 1038 residues of isoform 1 or 710 of isoform 2. The first 400 residues of the “short” isoform are essentially identical to the amino-terminal motor domain of isoform 1 and 2. Based on studies of Kif17 truncations, the short form of mouse Kif17 is predicted to form dimers and to bind microtubules.
*Compared to the reference sequence, this expression vector expresses a protein with substitution of conserved glycine-152 with a serine in the motor domain of Kif17. Due to this mutation, the properties of the produced protein could be altered.
Proper citation: RRID:Addgene_47545 Copy
Species: Caenorhabditis elegans
Genetic Insert: Glutamate-gated chloride channels
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2037; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23720773
Proper citation: RRID:Addgene_47542 Copy
Species: Homo sapiens
Genetic Insert: Rab35 Q67L
Vector Backbone Description: Vector Backbone:pcDNA3-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23264734
Proper citation: RRID:Addgene_47428 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pCFJ601; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23995389
Comments: For more information on Goldstein Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Goldstein/
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_47549 Copy
Species: Artemisia annua
Genetic Insert: ads
Vector Backbone Description: Backbone Marker:Mo Bi Tec; Backbone Size:7956; Vector Backbone:pHT01; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23483530
Proper citation: RRID:Addgene_47386 Copy
Species: Synthetic
Genetic Insert: SOX2 shRNA
Vector Backbone Description: Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941189
Proper citation: RRID:Addgene_47540 Copy
Species: Caenorhabditis elegans
Genetic Insert: glutamate-gated chloride Channels
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23720773
Proper citation: RRID:Addgene_47387 Copy
Species: Homo sapiens
Genetic Insert: Intersectin SH3 domain A
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47413 Copy
Species: Homo sapiens
Genetic Insert: cyclin E1
Vector Backbone Description: Backbone Size:6521; Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral, MSCV-IRES-GFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23873023
Comments: Human cyclin E1 (CCNE1) cDNA was mutated at N- and C-terminal Cdc4 phosphodegrons (T62A and T380A, see references) to disrupt interaction with Fbw7 ubiquitin ligase. cDNA is inserted into MIGR1 (MSCV-based retroviral vector), permitting transduction of hematopoietic cells.
Cloned cDNA contains approximately 100 bp from CCNE1 3' UTR.
GFP is co-expressed with cDNA via IRES, permitting enrichment for transduced cells using flow sorting.
References:
Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW et al (2001). Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase. Science 294: 173-177.
Strohmaier H, Spruck CH, Kaiser P, Won KA, Sangfelt O, Reed SI (2001). Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line. Nature 413: 316-322.
Proper citation: RRID:Addgene_47498 Copy
Species: Homo sapiens
Genetic Insert: Intersectin SH3 domain D
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47416 Copy
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