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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pRL-TK 4x wt
 
Resource Report
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10+ mentions
RRID:Addgene_11313 4 bulged binding sites for CXCR4 siRNA antisense Ampicillin PMID:15014042 Two original binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Flanking CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The inner binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:02:09 16
PAK4-FL-3'UTR
 
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1+ mentions
RRID:Addgene_113088 p21 Activated kinase 4 Homo sapiens Ampicillin PMID:29983868 Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin none 2026-08-15 01:02:08 1
pGL3 CXCR4 1P
 
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1+ mentions
RRID:Addgene_11310 1 perfect binding site for CXCR4 siRNA antisense Ampicillin PMID:12600936 The end of the ORF and the beginning of the 3' UTR is (with the TAA stop codon, followed by T, then the XbaI site TCTAGA): GATCGCCGTG TAA T TCTAGA GTCGGGGCG. We digested the plasmids with XbaI, and ligated in the following: ctaga ttccgagatatcggtaat gggcc. This produced a modified vector, still containing an XbaI site, but now also containing an ApaI site (GGGCCC), to allow for directional cloning of 3' UTR inserts. The final vector contains, starting with the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA. All UTR inserts were then constructed in the following format: TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC. A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG. The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU. Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:02:08 3
pcDNA3.1-GINKO1
 
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1+ mentions
RRID:Addgene_113112 GINKO1 Synthetic Ampicillin PMID:30652129 Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:08 2
pEYFP-N3-EPAC1
 
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1+ mentions
RRID:Addgene_113110 RapGEF3 Homo sapiens Kanamycin PMID:11801596 Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:02:08 1
Gata3-T2A-H2B-eGFP donor
 
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1+ mentions
RRID:Addgene_113119 Gata3 donor region including homology arms and T2A-H2B-eGFP Mus musculus Ampicillin PMID:29889212 Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:02:08 1
T777T
 
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1+ mentions
RRID:Addgene_113082 Ampicillin PMID:29924347 For plasmid usage, please see the associated publication: Sturm et al. Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516 Vector Backbone:T444T; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:02:08 2
KVS1-HA
 
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1+ mentions
RRID:Addgene_113061 K+ voltage sensitive channel subunit 1 Caenorhabditis elegans Ampicillin PMID:16222231 This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1. Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:08 1
pOTTC763 - pX458 with rat Rosa26 gRNA A
 
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1+ mentions
RRID:Addgene_113161 gRNA for rat Rosa26 Rattus norvegicus Ampicillin PMID:30792150 Note: Addgene NGS unable to fully resolve the CAG promoter sequence. Please refer to the depositor's provided sequence for that section of the plasmid. Backbone Marker:Feng Zhang; Backbone Size:9271; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:02:09 1
pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA
 
Resource Report
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1+ mentions
RRID:Addgene_113160 Kanamycin PMID:30792150 Backbone Marker:IDT; Backbone Size:1900; Vector Backbone:pIDTKan; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:02:09 1
pRL-TK let7 B
 
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1+ mentions
RRID:Addgene_11325 2 binding sites for let-7a miRNA Ampicillin PMID:15014042 2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Mutation B from figure 4A of paper. 2026-08-15 01:02:10 3
pEF6a-CD28-PafA
 
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1+ mentions
RRID:Addgene_113400 CD28-PafA unknown Ampicillin PMID:30104635 Vector Backbone:pEF6a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:11 1
pCasPA
 
Resource Report
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1+ mentions
RRID:Addgene_113347 Tetracycline PMID:30240613 Selectable Marker: Tetracycline; 30 μg/mL in E.coli and 100 μg/mL in Pseudomonas aeruginosa Backbone Size:17653; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:02:10 5
Membrane protein gRNA library
 
Resource Report
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1+ mentions
RRID:Addgene_113345 SAM sgRNA scaffold with MS2-hairpin loops Homo sapiens Ampicillin PMID:30477585 Vector Backbone:pKLV2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:02:10 2
pACRISPR
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_113348 Ampicillin PMID:30240613 Selectable Marker: Carbenicillin; 50 μg/mL in E.coli and 150 μg/mL in Pseudomonas aeruginosa Backbone Size:6936; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:02:10 3
pJY3919
 
Resource Report
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1+ mentions
RRID:Addgene_113332 beta-isopropyl malate dehydrogenase Yarrowia lipolytica Ampicillin PMID:29792930 Note: Plasmid contains a T base insertion near the C-terminus of the Leu2 marker. This insertion is not known to affect Leu2 function. Vector Backbone:pPS3911; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:10 1
2X PRE TK luc
 
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1+ mentions
RRID:Addgene_11350 PRE Homo sapiens Ampicillin PMID:10757795 Two copies of a consensus Progesterone Response Element (PRE) upstream of the thymidine kinase promoter. Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:02:12 2
pcDNA3.1 PA-mCit-GW
 
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1+ mentions
RRID:Addgene_113448 Chloramphenicol and Ampicillin PMID:29997244 Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:02:12 4
pMBP-FnCas12a
 
Resource Report
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1+ mentions
RRID:Addgene_113432 FnCas12a Ampicillin PMID:29449511 Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:12 2
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_113436 type II-C anti-CRISPR Haemophilus parainfluenzae Ampicillin PMID:30514786 Backbone Size:4094; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:02:11 1

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