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Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments:
Proper citation: RRID:Addgene_128073 Copy
Species: Synthetic
Genetic Insert: plum
Vector Backbone Description: Vector Backbone:pCDH-EF1-IRES cop GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128072 Copy
Species: Synthetic
Genetic Insert: ds red
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128067 Copy
Species: Synthetic
Genetic Insert: CMV promoter-EGFP
Vector Backbone Description: Vector Backbone:pShuttle; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_128065 Copy
Species: Synthetic
Genetic Insert: loxp-GFP-Zeo-Loxp
Vector Backbone Description: Vector Backbone:BS II KS+; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128064 Copy
Species: Synthetic
Genetic Insert: 10 amino acid glycine/serine linker + 5alanines
Vector Backbone Description: Backbone Size:3695; Vector Backbone:pB1H2w2-zif268 (ColE1); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30969105
Proper citation: RRID:Addgene_128165 Copy
Species: Synthetic
Genetic Insert: 10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
Vector Backbone Description: Backbone Size:3695; Vector Backbone:pB1H2w2-zif268 (ColE1); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30969105
Proper citation: RRID:Addgene_128164 Copy
Species: Synthetic
Genetic Insert: ERBIN PDZ domain
Vector Backbone Description: Backbone Size:7715; Vector Backbone:THE pGHUC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30969105
Proper citation: RRID:Addgene_128163 Copy
Species: Synthetic
Genetic Insert: hTetR::P2A::EGFP::T2A::PuroR
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7547; Vector Backbone:pDN-D2irTN2aG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a synthetic gene circuit controlling the PuroR gene using negative feedback through a humanized tetracycline repressor (hTetR) self-repressing a CMV-derived promoter with 2 tetO2 sites (CMV-D2ir). The circuit is also called mNF-PuroR.
Proper citation: RRID:Addgene_128255 Copy
Species: Synthetic
Genetic Insert: rtTA-Advanced::P2A::EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit that contains the regulator reverse tetracycline Trans Activator (rtTA) and the reporter EGFP, which is controlled by a tight TRE promoter that rtTA binds to activate its own expression in a positive feedback loop. The backbone consists of the Flp-In expression vector (pcDNA5/FRT). The synthetic gene circuit is also called mPF-GFP.
Proper citation: RRID:Addgene_128254 Copy
Species: Synthetic
Genetic Insert: Beta Globin Intron::hTetR::P2A::EGFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7505; Vector Backbone:pDN-D2irTNG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit with the humanized Tetracycline Repressor (hTetR) regulator separated from EGFP by a P2A motif, which is controlled by a CMV-derived promoter containing 2 tetO2 sites. With hTetR binding to the promoter, negative feedback lowers gene expression noise while linearizing the mean dose response. The circuit is also called mNF-GFP and is within a Flp-In expression vector.
Proper citation: RRID:Addgene_128253 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128198 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::nScCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128197 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::ScCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128192 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::xCas9 3.7:NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128191 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128196 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128189 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128222 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128221 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::LbCas12a (rice codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128187 Copy
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