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Species: E. coli
Genetic Insert: LacI-11/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90059 Copy
Species: E. coli
Genetic Insert: LacI/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90058 Copy
Species: E. coli
Genetic Insert: FruR (CRA)
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: WT FruR (CRA) . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90066 Copy
Species: E. coli
Genetic Insert: AOX1promoter - mazF - AOX1 tt- Zeocin R gene
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBluescript II KS(-); Vector Types:Gene insertion/deletion in Pichia pastoris; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19416369
Proper citation: RRID:Addgene_85073 Copy
Species: E. coli
Genetic Insert: E. coli MutL(R95F)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851738
Proper citation: RRID:Addgene_86608 Copy
Species: E. coli
Genetic Insert: PrhaBAD-sfGFP
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3822; Vector Backbone:pJ404; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247275
Proper citation: RRID:Addgene_87768 Copy
Species: E. coli
Genetic Insert: PrhaBAD-sfGFP
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3822; Vector Backbone:pJ404; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247275
Proper citation: RRID:Addgene_87767 Copy
Species: E. coli
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27060147
Proper citation: RRID:Addgene_78492 Copy
Species: E. coli
Genetic Insert: Mechanosensitive Channel of Small Conductance
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5364; Vector Backbone:pET28b; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446901
Proper citation: RRID:Addgene_78555 Copy
Species: E. coli
Genetic Insert: Chloramphenicol acetyltransferase
Vector Backbone Description: Backbone Marker:natural plasmid; Backbone Size:60000; Vector Backbone:RP4; Vector Types:Helper plasmid for mobilization; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:15212888
Comments: Depositor states that users can select for the chromosome with nalidixic acid.
Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity
Proper citation: RRID:Addgene_79811 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27284167
Proper citation: RRID:Addgene_79879 Copy
Species: E. coli
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_82037 Copy
Species: E. coli
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_82040 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_80579 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80582 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tmos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_80580 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80583 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91229 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91227 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91244 Copy
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