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Species: Homo sapiens
Genetic Insert: PARP1-W318R
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (W318R, V762A) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174793 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A762V
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (see PMID: 20399804 for detail about the mutation)
Proper citation: RRID:Addgene_174795 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A774L
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, A774L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174796 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A870L
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, A870L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174797 Copy
Species: Homo sapiens
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:AAVS1-TRE3G-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM604, contains homology arms for HDR integration into human AAVS1 locus . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174707 Copy
Species: Homo sapiens
Genetic Insert: Lenti-evoA1-BE4max
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34663942
Proper citation: RRID:Addgene_174700 Copy
Species: Homo sapiens
Genetic Insert: Lenti-evoA1-seBEN
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34663942
Comments: 5' Cloning Site: BsmbI (unknown if destroyed), 3' Cloning Site: BsmbI (unknown if destroyed).
Proper citation: RRID:Addgene_174701 Copy
Species: Homo sapiens
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:AAVS1-TRE3G-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM603, contains homology arms for HDR integration into human AAVS1 locus . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174706 Copy
Species: Homo sapiens
Genetic Insert: P100
Vector Backbone Description: Vector Backbone:pCLXSN(GFP); Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174734 Copy
Species: Homo sapiens
Genetic Insert: p100 SS/AA
Vector Backbone Description: Vector Backbone:pCLXSN(GFP); Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174735 Copy
Species: Homo sapiens
Genetic Insert: P100
Vector Backbone Description: Vector Backbone:pCLXSN(GFP); Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086255
Proper citation: RRID:Addgene_174738 Copy
Species: Homo sapiens
Genetic Insert: OTUB1
Vector Backbone Description: Vector Backbone:pPRIChp; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31182807
Proper citation: RRID:Addgene_174743 Copy
Species: Homo sapiens
Genetic Insert: ALC1-K77R
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-897 (a start codon included in-frame)
Please note: Plasmid contains R25P and S743C natural variants in ALC1.
Proper citation: RRID:Addgene_174813 Copy
Species: Homo sapiens
Genetic Insert: ALC1-E175Q
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-897 (a start codon included in-frame).
Please note: Plasmid contains R25P and S743C natural variants in ALC1.
Proper citation: RRID:Addgene_174814 Copy
Species: Homo sapiens
Genetic Insert: PARP1-G972R
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, G972R) (see PMID: 9315851 for detail about the mutation)
Proper citation: RRID:Addgene_174800 Copy
Species: Homo sapiens
Genetic Insert: PARP1-Y986H
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, Y986H) (see PMID: 9315851 for detail about the mutation)
Proper citation: RRID:Addgene_174801 Copy
Species: Homo sapiens
Genetic Insert: PARP1-Y986S
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, Y986S) (see PMID: 9315851 for detail about the mutation)
Proper citation: RRID:Addgene_174802 Copy
Species: Homo sapiens
Genetic Insert: PARP1deltaART
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-784 (V762A)
Proper citation: RRID:Addgene_174804 Copy
Species: Homo sapiens
Genetic Insert: PE2-P2A-hMLH1dn
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34653350
Proper citation: RRID:Addgene_174827 Copy
Species: Homo sapiens
Genetic Insert: Specificity Protein 1
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:14873; Vector Backbone:pLentiCRISPRv2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33730584
Proper citation: RRID:Addgene_174907 Copy
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