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Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128220 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::FnCas12a (rice codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128186 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 3
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128226 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128223 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 5
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128228 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for tRNA-gRNA array, Position 4
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128227 Copy
Species: Synthetic
Genetic Insert: 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128181 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::SpCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128180 Copy
Species: Synthetic
Genetic Insert: AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128183 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128211 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128210 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 2
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128215 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128214 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128213 Copy
Species: Synthetic
Genetic Insert: sgRNA#1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243081
Proper citation: RRID:Addgene_128179 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 5
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128218 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 4
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128217 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128173 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128171 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Proper citation: RRID:Addgene_128167 Copy
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