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Species: Other
Genetic Insert: Intersectin SH3 domain A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47396 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pCFJ601; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23995389
Comments: For more information on Goldstein Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Goldstein/
gRNA target sequence atatcagtctgtttcgtaa
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_47550 Copy
Species: Mus musculus
Genetic Insert: Nanog
Vector Backbone Description: Vector Backbone:pCR2.1TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23992847
Proper citation: RRID:Addgene_48680 Copy
Species: Homo sapiens
Genetic Insert: Lacritin
Vector Backbone Description: Backbone Marker:New England BioLabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23640897
Comments: Please note that amino acid numbering of this plasmid is relative to amino acid #20 in GenBank reference sequence NP_150593.1. E20 in the reference sequence is considered the first amino acid of lacritin in this plasmid.
Lacritin protein sequence in this plasmid is: AAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKAGKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA
Proper citation: RRID:Addgene_48718 Copy
Species: Mus musculus
Genetic Insert: ULK1
Vector Backbone Description: Backbone Marker:Sabatini lab; Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23888043
Proper citation: RRID:Addgene_48799 Copy
Species: Homo sapiens
Genetic Insert: YWHAZ
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12757707
Proper citation: RRID:Addgene_48798 Copy
Species: N. meningitidis
Genetic Insert: Cas9-VP64, nuclease-null
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3 TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48676 Copy
Species: Homo sapiens
Genetic Insert: YWHAE 14-3-3 ϵ
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12757707
Proper citation: RRID:Addgene_48797 Copy
Species: N. meningitidis
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3 hygro; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48670 Copy
Species: S. pyogenes
Genetic Insert: Cas9-VP64, nuclease-null
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3 TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48674 Copy
Species: Mus musculus
Genetic Insert: RORb
Vector Backbone Description: Vector Backbone:CBIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence from the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_48709 Copy
Species: S. thermophilus #1
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3 TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48669 Copy
Species: Synthetic
Genetic Insert: 35S promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
35S promoter = 2252-3115bp
BsaI site #2 = 3116-3126bp
Proper citation: RRID:Addgene_48815 Copy
Species: Arabidopsis thaliana
Genetic Insert: WUS promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
WUS promoter = 2252-6681bp
BsaI site #2 = 6682-6692bp
Proper citation: RRID:Addgene_48814 Copy
Species: Arabidopsis thaliana
Genetic Insert: AP3 promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
AP3 promoter = 2252-3526bp
BsaI site #2 = 3527-3537bp
Proper citation: RRID:Addgene_48813 Copy
Species: Mus musculus
Genetic Insert: nAChr-alpha6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17932221
Proper citation: RRID:Addgene_48812 Copy
Species: Mus musculus
Genetic Insert: nAChr Beta 3 subunit, isoform 1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5597; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17932221
Comments: *Compared to the NCBI Genbank Entrez entry for nAChr-beta3, this plasmid has a K107R amino acid residue substitution. See accession ID: AAL75573.1|AF467896_1 for the correct reference sequence.
Proper citation: RRID:Addgene_48811 Copy
Species: Synthetic
Genetic Insert: 8xLexA-binding elements
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2736; Vector Backbone:pMD20; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22043306
Proper citation: RRID:Addgene_48807 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23888043
Proper citation: RRID:Addgene_48806 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23888043
Proper citation: RRID:Addgene_48805 Copy
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