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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGBW-m4046533
 
Resource Report
Resource Website
RRID:Addgene_145537 SARS-CoV-2 nsp11 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725312.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046363
 
Resource Report
Resource Website
RRID:Addgene_145532 SARS-CoV-2 nsp8 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725304.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046522
 
Resource Report
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RRID:Addgene_145530 SARS-CoV-2 2'-O-ribose methyltransferase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725311.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046475
 
Resource Report
Resource Website
RRID:Addgene_145531 SARS-CoV-2 N (nucleocapsid) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724397.2Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046446
 
Resource Report
Resource Website
RRID:Addgene_145494 SARS-CoV-2 nsp11 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725312.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
pGBW-m4046364
 
Resource Report
Resource Website
RRID:Addgene_145491 SARS-CoV-2 2'-O-ribose methyltransferase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725311.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
pGBW-m4046541
 
Resource Report
Resource Website
RRID:Addgene_145492 SARS-CoV-2 leader protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725297.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
pGBW-m4046216
 
Resource Report
Resource Website
RRID:Addgene_145490 SARS-CoV-2 M (membrane) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724393.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:56 0
pGBW-m4046593
 
Resource Report
Resource Website
RRID:Addgene_145539 SARS-CoV-2 ORF6 protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724394.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046347
 
Resource Report
Resource Website
RRID:Addgene_145546 SARS-CoV-2 nsp9 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pJRL2 PHO5pr VYFP (EB#1547)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14840 PHO5 promoter Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:08 1
pnEA-NpM-ScEap1p_91-150_AF
 
Resource Report
Resource Website
RRID:Addgene_148710 ScEap1p_91-150 Saccharomyces cerevisiae Ampicillin PMID:30053226 Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:09 0
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14846 PHO5 promoter TATA-A1T6 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-A1T6. TATA box mutated from TATATAAG to AATATTAG. 2026-08-15 01:07:08 1
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)
 
Resource Report
Resource Website
RRID:Addgene_14844 PHO5 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-08-15 01:07:09 0
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)
 
Resource Report
Resource Website
RRID:Addgene_14849 GAL1 promoter TATA-A1T6 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-A1T6. TATA box mutated from TATATAAG to AATATTAG. 2026-08-15 01:07:08 0
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)
 
Resource Report
Resource Website
RRID:Addgene_14850 GAL1 promoter TATA-A1T6 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-A1T6. TATA box mutated from TATATAAG to AATATTAG. 2026-08-15 01:07:08 0
pGBW-m4046464
 
Resource Report
Resource Website
RRID:Addgene_148970 SARS-CoV-2 S (surface glycoprotein) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724390.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:09 0
pGBW-m4046258
 
Resource Report
Resource Website
RRID:Addgene_148973 SARS-CoV-2 RNA-dependent RNA polymerase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:09 0
pGBW-m4046227
 
Resource Report
Resource Website
RRID:Addgene_148975 SARS-CoV-2 ORF7a protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724395.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:09 0
pGBW-m4046574
 
Resource Report
Resource Website
RRID:Addgene_148976 SARS-CoV-2 S (surface glycoprotein) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724390.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:09 0

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